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Subject: Chemistry × Author: Jain, Jigyasa × End date: 2009 × Start date: 2004 ×
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    ThesisItemOpen Access
    Effects of Pongamia pinnata extracts on phytopathogenic fungi and characterisation of active components
    (CCSHAU, 2008) Jain, Jigyasa; Rajvir Singh
    Pongamia pinnata is an avenue tree, belonging to family Leguminosae and sub family papilionoideae, distributed throughout India. The plant is known for its anti-inflammatory, analgesic, antipyretic and anti-ulcer activity. Plant of P.pinnata was collected from different places, CCS Haryana Agricultural University, Hisar. Its leaves and seeds were shadow dried and then extracted with hot methanol. Colomn chromatography of the extract over silica gel afforded Five compounds. Four of these, Nonacosane, Methyl hexatriacontanoate, Tritracontane and Pentacosanoic acid were already reported. The compound 22, 29, 30-Trisnor-(17αH)-Hopane has been reported for the first time from P. pinnata. Isolated compounds were characterized on the basis of IR, 1H-NMR, ii mass spectral data and other properties of the compounds. The identities of some of the compounds were confirmed by direct comparisons with the authentic samples. The hexane, chloroform, acetone fractions and methanolic extract of leaves and seeds of P. pinnata were evaluated for the antifungal activity against Fusarium oxysporum and Colletotrichum falcatum by poisoned food technique. The extracts each in 500, 1000, 1500 and 2000 μg/ml concentrations were tested against F. oxysporum and C. falcatum and percentage inhibition was recorded. The mycelial growth of F. oxysporum was inhibited to the maximum extent by chloroform fraction with 47% inhibition followed by hexane and methanolic extract 43% at 2000 μg/ml concentration. Chloroform fraction was found active having the percentage inhibition 50, hexane and acetone fractions with 41 and 38 against F. oxysporum at 2000 μg/ml concentration. It may be concluded that F. oxysporum fungus is more susceptible to these extract/fractions.