Effects of Pongamia pinnata extracts on phytopathogenic fungi and characterisation of active components
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Date
2008
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CCSHAU
Abstract
Pongamia pinnata is an avenue tree, belonging to family Leguminosae and
sub family papilionoideae, distributed throughout India. The plant is known for its
anti-inflammatory, analgesic, antipyretic and anti-ulcer activity. Plant of
P.pinnata was collected from different places, CCS Haryana Agricultural
University, Hisar. Its leaves and seeds were shadow dried and then extracted with
hot methanol. Colomn chromatography of the extract over silica gel afforded
Five compounds. Four of these, Nonacosane, Methyl hexatriacontanoate,
Tritracontane and Pentacosanoic acid were already reported. The compound 22,
29, 30-Trisnor-(17αH)-Hopane has been reported for the first time from P.
pinnata. Isolated compounds were characterized on the basis of IR, 1H-NMR,
ii
mass spectral data and other properties of the compounds. The identities of some
of the compounds were confirmed by direct comparisons with the authentic
samples.
The hexane, chloroform, acetone fractions and methanolic extract of leaves
and seeds of P. pinnata were evaluated for the antifungal activity against
Fusarium oxysporum and Colletotrichum falcatum by poisoned food technique.
The extracts each in 500, 1000, 1500 and 2000 μg/ml concentrations were tested
against F. oxysporum and C. falcatum and percentage inhibition was recorded.
The mycelial growth of F. oxysporum was inhibited to the maximum extent by
chloroform fraction with 47% inhibition followed by hexane and methanolic
extract 43% at 2000 μg/ml concentration. Chloroform fraction was found active
having the percentage inhibition 50, hexane and acetone fractions with 41 and 38
against F. oxysporum at 2000 μg/ml concentration. It may be concluded that
F. oxysporum fungus is more susceptible to these extract/fractions.
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Keywords
Hybrids, Cotton, Sowing, Crossing over, Heterosis, Yields, Planting, Additives, Developmental stages, Beverages