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Subject: Biotechnology and Molecular Biology × Author: Bhanakher, Ajay Kumar × End date: 2009 × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Micro propagation of some important cultivars of strawberry
    (CCSHAU, 2008) Bhanakher, Ajay Kumar; Jain, R.K.
    Strawberry (Fragaria × ananassa Duch) is one of the most important berry crop, which is cultivated throughout the world from artic to tropics. Conventionally strawberry is propagated by runners. Non availability of elite disease-free plant material has been recognized as the major barrier in expanding its cultivation. Micropropagation can be a potential tool in this direction to produce true-to-type, disease free and quality plants throughout the year. In this project, experiments were conducted to develop an efficient protocol for in vitro shoot induction/multiplication, rooting and transplantation in three cultivars of strawberry, Sweet Charlie, Ofra and Camarosa. Of the 13 surface sterilization procedures assessed, S4 and S9 disinfection treatments that involved the Bavistin treatment of plants foliage before culture as well as of explants during the surface sterilization procedure were most effective in strawberry cvs. Sweet Charlie and Ofra. Proliferation from shoot tip explants of 2-5 mm in length was observed within 7-12 days of culture. In all the three cultivars, maximum number (5-15) of shoots per explant was obtained in MS3 medium containing IAA and BAP (1.5 μm and 5 μm respectively). Maximum shoot multiplication frequency was obtained in cultivar Sweet Charlie followed by Ofra and Camarosa. In cv. Ofra, shoots when left little longer (> one month) in the same medium, showed rooting. Transplantation procedure which involved the washing of plants with running tap water, transplantation in pots containing a mixture of cocopit, vermiculite and perlite in 3:1:1 ratio and covering of the plants with polythene bags, was highly successful. The two micropropagated plants of cultivar Ofra survived well after transplantation. After transplantation, both the micropropagated plants looked normal with no morphological changes in leaf structure and plant type. The micropropagated plants started producing runner one month after transplantation. Frequency of runner production was 10-15 per plant after three months of transplantation. One of the two micropropagated plant showed flowering and fruit establishment.