Micro propagation of some important cultivars of strawberry
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Date
2008
Authors
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Publisher
CCSHAU
Abstract
Strawberry (Fragaria × ananassa Duch) is one of the most
important berry crop, which is cultivated throughout the world from artic to
tropics. Conventionally strawberry is propagated by runners. Non availability
of elite disease-free plant material has been recognized as the major barrier in
expanding its cultivation. Micropropagation can be a potential tool in this
direction to produce true-to-type, disease free and quality plants throughout
the year. In this project, experiments were conducted to develop an efficient
protocol for in vitro shoot induction/multiplication, rooting and
transplantation in three cultivars of strawberry, Sweet Charlie, Ofra and
Camarosa. Of the 13 surface sterilization procedures assessed, S4 and S9
disinfection treatments that involved the Bavistin treatment of plants foliage
before culture as well as of explants during the surface sterilization procedure
were most effective in strawberry cvs. Sweet Charlie and Ofra. Proliferation
from shoot tip explants of 2-5 mm in length was observed within 7-12 days of
culture. In all the three cultivars, maximum number (5-15) of shoots per
explant was obtained in MS3 medium containing IAA and BAP (1.5 μm and 5
μm respectively). Maximum shoot multiplication frequency was obtained in
cultivar Sweet Charlie followed by Ofra and Camarosa. In cv. Ofra, shoots
when left little longer (> one month) in the same medium, showed rooting.
Transplantation procedure which involved the washing of plants with running
tap water, transplantation in pots containing a mixture of cocopit, vermiculite
and perlite in 3:1:1 ratio and covering of the plants with polythene bags, was
highly successful. The two micropropagated plants of cultivar Ofra survived
well after transplantation. After transplantation, both the micropropagated
plants looked normal with no morphological changes in leaf structure and
plant type. The micropropagated plants started producing runner one month
after transplantation. Frequency of runner production was 10-15 per plant
after three months of transplantation. One of the two micropropagated plant
showed flowering and fruit establishment.
Description
Keywords
Planting, Strawberry (plant), Micropropagation, Irrigation, Regeneration, Biological phenomena, Biological development, Tissue culture, Planting equipment, Vegetative propagation