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20242
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Subject: Veterinary Gynaecology and Obstetrics × End date: 2024 × Start date: 2024 ×
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    ThesisItemOpen Access
    EVALUATION OF ESTRUS PARAMETERS, CERTAIN BIOCHEMICAL AND HORMONAL PROFILES IN REPEAT BREEDING ONGOLE COWS
    (SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502. (A.P.) INDIA, 2024-02) UDAYA KIRAN ALLADA; CHANDRA PRASAD .B (MAJOR); SUNNY PRAVEEN .K; KAMALAKAR .G
    The present study was conducted in follicular cyst affected Ongole cows, which were presented to the Gynaecological unit, Department of Veterinary Gynaecology and Obstetrics, NTR College of Veterinary Science, Gannavaram, Andhra Pradesh during the study period from November 2022 to October 2023. A thorough analysis was conducted on both day 0 and day 10 in normal cyclic (n=10) and follicular cyst affected Ongole cows (n=10). Various parameters including estrus characteristics, haemato-biochemical values, and hormonal profiles were meticulously examined, recorded and subsequently compared between the groups. The prevalence of cystic ovaries in Ongole cattle experiencing repeated breeding was recorded as 21.3 per cent, with follicular cysts comprising 13.3 per cent of the cases. In the current study, the occurrence of follicular cysts in Ongole cows was 20 per cent in second parity, 30 per cent in third parity and 50 per cent in four and above parities, respectively. Results revealed that duration of estrus (51.9 hrs) was significantly (P<0.01) longer and estrous cycle length (15.9 days) was significantly (P<0.01) shorter in follicular cystic compared to normal cyclic cows. Further, most of the follicular cystic cows exhibited an intense estrus compared to intermediate estrus in normal cyclic cows. The majority of follicular cystic cows exhibited a pH ranging between 7-8 with a non-significantly (P>0.05) lower spinbarkeit value and typical fern pattern in their cervico-vaginal mucus. Further, they displayed clinical signs like nymphomania (40%), sterility hump (60%), mucometra (30%), copious vaginal discharges (70%), pneumovagina (40%) and estrual prolapse (20%). On day 0, the average diameter (cm) of dominant and cystic follicles in normal cyclic and follicular cystic cows was 1.06±0.06 and 1.86±0.07 cm, respectively. Meanwhile, the mean diameter of cystic follicles on day 10 was 1.93±0.07. Significantly (P<0.05) lower levels of haemoglobin (Hb), packed cell volume (PCV) and slight leukocytopenia with neutrophilia, lymphopenia, mild monocytopenia and a non-significant variation in basophil count were recorded in follicular cystic compared to normal cyclic cows on both days. Significantly (P<0.05) higher serum glucose, total protein and a non significantly (P>0.05) higher serum cholesterol concentration was recorded in cows afflicted with follicular cysts compared to normal cyclicity on both days. A significantly (P<0.05) higher and lower levels of serum progesterone on day 0 and day 10, respectively was observed in follicular cystic compared to normal cyclic cows. Whereas, significantly (P<0.05) higher serum cortisol level was recorded in follicular cystic compared to normal cyclic cows on both days. A significant (P<0.01) rise in serum progesterone level was noted in normal cyclic cows on day 10 in contrast to day 0 with no significant difference in follicular cystic cows. While, a non-significant decrease (P>0.05) in serum cortisol was noted in normal cyclic cows on day 10 compared to day 0, with no significant variation observed in follicular cystic cows.
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    ThesisItemOpen Access
    COMPARATIVE EFFICACY OF OPU AND IN-VITRO EMBRYO PRODUCTION BETWEEN GIR AND ONGOLE (Bos indicus) DONORS
    (SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502. (A.P.) INDIA, 2024-01) RAJ KUMAR RAGALA; MUTHA RAO .M (MAJOR); SRINIVAS MANDA; SRINIVASA PRASAD .CH
    Ultrasound guided transvaginal follicular aspiration (TVFA), also known as ovum pick up (OPU) to collect oocytes from live cows for in-vitro embryo production (IVEP) has opened up greater possibilities to exploit productive and reproductive potential of the valuable donor. The present study was aimed to compare the efficacy of ovum pick-up (OPU) and In-vitro embryo production rate between Ongole and Gir breed cows. Gir (n=6) and Ongole (n=12) cows were assigned to group 1 and group 2, respectively and all the animals were subjected to FSH pre-stimulation before OPU. The mean number of follicles available for aspiration (43.89 ± 5.38 vs 41.58 ± 5.97) did not vary between groups. Size distribution of follicles indicate that small (< 4mm) and large (>8 mm) sized follicles were lower and medium (4-8 mm) sized follicles were higher in both the groups. The mean number of follicles aspirated (34.17 ± 4.80 – group 1 vs. 27.42 ± 5.09 – group 2) and the COCs recovered (18.17 ± 5.90 – group 1 vs. 16.0 ± 3.90 – group 2) did not vary between groups. A total of 109 and 192 COCs were recovered in group 1 and 2, respectively. Of the total COCs recovered, higher percentage was of viable in group 1 (100%) than in group 2 (90.63%), but the difference was not significant (P>0.05). However, significantly lower percentage of non-viable COCs were obtained in group 1 (0%) than in group 2 (9.37%). The overall mean blastocyst development rate was non significantly higher in group 1 (52.15 %) than group 2 (35.82%). The mean number of embryos produced per cow was 11.33 ± 4.99 in group 1 and 6.50 ± 2.26 in group 2. It was concluded that the efficacy of OPU was not affected by breed as both Gir and Ongole belong to Bos indicus category with similar follicular population available at aspiration. However, higher viable oocyte yield in Gir breed eventually resulted in more number of blastocysts per OPU in this breed.