COMPARATIVE EFFICACY OF OPU AND IN-VITRO EMBRYO PRODUCTION BETWEEN GIR AND ONGOLE (Bos indicus) DONORS
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Date
2024-01
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SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI - 517 502. (A.P.) INDIA
Abstract
Ultrasound guided transvaginal follicular aspiration (TVFA), also known as ovum
pick up (OPU) to collect oocytes from live cows for in-vitro embryo production (IVEP)
has opened up greater possibilities to exploit productive and reproductive potential of the
valuable donor.
The present study was aimed to compare the efficacy of ovum pick-up (OPU) and
In-vitro embryo production rate between Ongole and Gir breed cows. Gir (n=6) and
Ongole (n=12) cows were assigned to group 1 and group 2, respectively and all the
animals were subjected to FSH pre-stimulation before OPU. The mean number of follicles
available for aspiration (43.89 ± 5.38 vs 41.58 ± 5.97) did not vary between groups. Size
distribution of follicles indicate that small (< 4mm) and large (>8 mm) sized follicles
were lower and medium (4-8 mm) sized follicles were higher in both the groups. The
mean number of follicles aspirated (34.17 ± 4.80 – group 1 vs. 27.42 ± 5.09 – group 2) and
the COCs recovered (18.17 ± 5.90 – group 1 vs. 16.0 ± 3.90 – group 2) did not vary
between groups. A total of 109 and 192 COCs were recovered in group 1 and 2,
respectively. Of the total COCs recovered, higher percentage was of viable in group 1
(100%) than in group 2 (90.63%), but the difference was not significant (P>0.05).
However, significantly lower percentage of non-viable COCs were obtained in group 1
(0%) than in group 2 (9.37%). The overall mean blastocyst development rate was non
significantly higher in group 1 (52.15 %) than group 2 (35.82%). The mean number of
embryos produced per cow was 11.33 ± 4.99 in group 1 and 6.50 ± 2.26 in group 2. It
was concluded that the efficacy of OPU was not affected by breed as both Gir and Ongole
belong to Bos indicus category with similar follicular population available at aspiration.
However, higher viable oocyte yield in Gir breed eventually resulted in more number of
blastocysts per OPU in this breed.