Browsing by Author "Sujatha, P.L."
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ArticleItem Open Access Analysis Of the Mechanism Of Action By Molecular Docking Studies Of One Ethno Veterinary Herbal Preparation Used In Bovine Mastitis(2017-08) Punniamurthy, N.; Sujatha, P.L.; Preetha, S.P.; Ramakrishnan, N.; TANUVASMastitis, a disease in dairy cattle characterized by inflammation due, but not necessarily limited, to microbial infection in the mammary gland. Mastitis leads to loss in terms of reduction in milk, milk discards, early culling, increasing labour cost and veterinary services. The mammary tissue damage decreases the number and the activity of epithelial cells, caused by bacterial factors and host immune system through necrosis or apoptosis, which can be differentiated by the changes in morphological, biochemical and molecular characters of the dying cells. However, bacteria and their products contribute to the initial development of the disease. Staphylococcus aureus is a pathogen with a broad range of hosts and mastitis is a major disease caused by it. It has the ability to colonize the host tissue, causing more acute relapsing infection than other staphylococcus species. Many strategies have been employed in the treatment among which the topical application of herbal paste comprising Aloe Vera, turmeric powder and lime has been effective against the infection though the mechanism of action is not fully understood. The present study uses the in silico approach to find the effect of the herbal preparation against the infection. The bioactive compounds were tested for its effect against the target proteins of S. aureus using molecular docking studies.Training ManualItem Open Access Bioinformatics and its Applications in Animal Health and Veterinary Research - September 2009(TANUVAS, Chennai, 2009-09) Dhanapalan, P.; Kumanan, K.; Devendran, P.; Selvaraj, P.; Sujatha, P.L.; TANUVASBook chapterItem Open Access BIOLOGICAL INFORMATION MANAGEMENT (BIM)(TANUVAS, Chennai, 2009-09) Sujatha, P.L.; TANUVASInformation is a major organizational resource. information is the result of processing, manipulating and organizing data in a way that adds to the knowledge of the receiver. Up-to-date information at the correct time is very much essential for the development of any nation. Information is available in different forms like journal articles, conference proceedings, books, reviews, reports, patents etc., Due to the growth of information technology, most of the documents are available in both print as well as e-formats.ThesisItem Open Access Comparative Analysis of EST Sequences of Different Plant Genomes(TANUVAS, 2005) Yamuna, R.; TANUVAS; Thulasi, G.; Raj, G. Dhinakar; Sujatha, P.L.ThesisItem Open Access Comparative Modelling of Human erb B4 Protein(TANUVAS, 2005) Hemalatha, C.R.; TANUVAS; Thulasi, G.; Ramadass, P.; Sujatha, P.L.ArticleItem Open Access A Computational Approach to use the Active Compounds in Rosemary (Rosmarinusofficinalis), Responsible for its Anti-Inflammatory Properties, as Potential Ligands in Non-Steroidal Anti-Inflammatory Drugs (NSAIDs)(2014-06) Hari, Archana; Kumarasamy, P.; Sujatha, P.L.; Sureshkannan, S.; Priya, Sankar; TANUVASRosemary is a woody, perennial herb. The herb is known to induce relaxation of the bronchial smooth muscle and intestinal smooth muscle along with reduction in leukotrienes and increased PGE2 production, inhibition of lipid peroxidation, and inhibition of the complement. It is particularly known to have anti-oxidative and anti-inflammatory properties. Inflammatory abnormalities are a large group of disorders that underlie a variety of human diseases. The immune system is often involved with inflammatory disorders, demonstrated in both allergic reactions and some myopathies, with many immune system disorders resulting in abnormal inflammation. The following docking study was performed on the active ingredients in Rosemary that give the herb its anti-inflammatory property. The dock scores were analysed and the best active ingredient-target interactions identified.ArticleItem Open Access A computational approach to use the active principles in Custard apple (Annona squamosa), as an ectoparasiticide by using potential target Acetylcholinesterase of Rhipicephalus sanguineus (Brown dog tick)(2016) Pavithra, S.; Preetha, S.P.; Sujatha, P.L.; Srinivasan, M.R.; Karthikeyan, A.; Balachandran, C.; Venkateswaran, K.V.; Kumarasamy, P.; TANUVASRhipicephalus sanguineus is a brown dog tick that transmits rickettsial infection between dogs and man. It can be prevented by using ectoparasiticides generally acting through acetylcholinesterase inhibiting mechanism which is responsible for the catalysis of acetylcholine neurotransmitter. To overcome the toxic effects produced by synthetic ectoparasticides, new in silico approaches were carried out using the active principles from the custard apple. Custard apple is a small tree cultivated throughout India with various medicinal properties. A novel docking study was performed using the active ingredients present in the leaves of the custard apple. The dock scores were analysed and the best active ingredient-target interactions were identified. The active principle Norcorydine gives good docking scores with Acetylcholinesterase. Hence the present study projects the positive role of custard apple in the synthesis of bio-insecticides for the control of ectoparasites.ArticleItem Open Access Computational Prediction and In Silico Analysis of Gyrase Gene QRDR Region Coding Drug Resistance among Mycobacterium Tuberculosis Isolates of India(aizeon publishers, 2014) Sasikalaveni, A.; Sujatha, P.L.; Parthiban, M.; Dhanapalan, P.; Aruni, A. Wilson; TANUVASThe Gyrase A gene of Mycobacterium tuberculosis has been shown to be responsible for the quinolone drug resistance. The Gyrase gene sequences we submitted in NCBI were retrieved. Accession No: EU835533, EU835534, EU835535. (Mtb1, Mtb2, Mtb3) and BLAST analysis revealed 95% identity with the available Mycobacterium tuberculosis Gyrase A gene. These sequences were also compared with the available Mycobacterium Gyrase gene of Indian isolates. The Gyrase A proteins were modelled using Modeller 9v3 version and the Ramachandran plot evaluation of all the models showed 94% in favourable region. The resistant strain (EU512947) reported from Coimbatore was also modelled and the three dimensional structure comparison of Mtb1, Mtb2, Mtb3 with the resistant Coimbatore isolate Gyrase coding protein was done using STRAP. The molecular similarity of the GyraseA protein of Mtb3 and EU512947 suggests all possible means of conversion this strain to a resistant Mycobacterial species.ArticleItem Open Access Computer-Aided Approach to Study the Anti- Tumour Potentials of Seaweed against Canine Breast Cancer(Krishi Sanskriti Publications, 2016-07) Sujatha, P.L.; Preetha, S.P.; Balachandran, C.; Kumarasamy, P.; Venkateswaran, K.V.; Devaraj, H.; TANUVASlmong all the type of cancer, the incidence of breast cancer in canines has been increasing despite the J-act that the elforts " to prevent the disease has been improving. The traditional chemotherapy has been shown to be elfective though it exhibit some serious adverse effects as the targets of the chemotherapeutic agents are nonspecific in most cases. Hence the chemotherapeutic agents affect the normal cells leading to the adverse fficts. Howetter researchers have been working for the past two decades to find the alternative treatment to the cancer with lesser adverse ffict. Natural products or the bioactive compounds from natural sources have been proved to be less toxic. Fucoidan is one such natural polysaccharide of sulphated fucose residues from seaweed. Fucoidan have been used in cancer treatment in many countries. In this study we analyse the ability offucoidan to treat breast cancer using in silico approaches.Book chapterItem Open Access DATA MINING FOR DRUG DISCOVERY(TANUVAS, Chennai, 2009-09) Dhanapalan, P.; Sujatha, P.L.; Devendran, P.; TANUVASData mining sifts through huge amounts of information and extracts previously unrealised information about molecular compounds, product portfolio, clinical studies, and customer information that can be applied to accelerate drug discovery and development. For example, you could match desirable biochemical specifications with disease. and patient characteristics, and select compounds with the highest probability of a favourable safety and efficacy profile.ArticleItem Open Access Designing of Antiviral Sirna for Targeting HIV-1 Subtype C in India(2014-10) Samantha, E. Aashmi; Dhanapalan, P.; Kumarasamy, P.; Sujatha, P.L.; Devendran, P.; Sureshkannan, S.; TANUVASAcquired Immunodeficiency Syndrome (AIDS) is one of the top deadly diseases in the world. The recent studies confirm that neither behavioral therapies nor current antiretroviral drugs are likely to cure HIV infection completely. RNA interference (RNAi) has raised the possibility of developing a new generation of strategies that can prevent HIV-1 replication. Since HIV has high mutation rate, RNAican be made effective only by targeting highly conserved coding regions with optimal stable siRNAs. This study was mainly focused on the identification of highly conserved target sites and designing of stable antiviral siRNAs against HIV-1 subtype C in India. Here, the mutations in HIV-1 subtype C in India were studied using the tool HIVseq. The evolutionary pathway of HIV-1 subtype C in India was inferred through phylogenetic analysis using MEGA4 in the present study. Based on the mutational and phylogenetic studies, the highly conserved target sites were identified using BioEdit. Stable, optimal siRNAs were designed against these conserved target sites using siRNA selection server at Whitehead Institute. siRNAs designed against these conserved target sites were anticipated to resist viral mutational escape and off-target gene silencing. These optimal siRNAs would increase the durability of RNAi against HIV-1 subtype C.ArticleItem Open Access Evaluation of Antitumor Potentials of Herbal Based Natural Products in Experimentally Induced Carcinogenesis In Silico and In Vitro(2016) Preetha, S.P.; Punniamurthy, N.; Balachandran, C.; Cecilia, J.; Mangalagowri, A.; Sureshkannan, S.; Sujatha, P.L.; TANUVASCancer has emerged as one of the most dreaded diseases of the 20th century, spreading further with continuance and increasing incidence in 21stcentury. Though chemoprevention has evolved as a promising and valuable strategy to inhibit, suppress or control the incidence of carcinogenesis, it is essential to explore the chemotherapeutic agents in experimental models before embarking on clinical trials. Experimental tumours have great importance for the purposes of modelling and Ehrlich Ascites Carcinoma (EAC) is one of the commonest since it is an undifferentiated carcinoma with rapid proliferation, high transplantable capability and bears similarities to human tumours. Natural products play a pivotal role in cancer therapy with substantial numbers of anticancer agents being natural or natural derived products with negligible side effects. The use of herbal based natural products may present an affordable and sustainable alternative to synthetic medicines. Acalyphaindica Linn. andTinosporacordifolia are used in Traditional System of Medicine and ethnoveterinary practices with multiple pharmacological activities. Keeping in view the importance and advantages of combination chemotherapy using herbal based natural products on the tumour cells, this study was attempted to explore the influence of combined extracts of Acalyphaindica and Tinosporacordifolia in experimentally induced carcinogenesis invitro. The utility and impact of herbal based natural products, the combined extracts of AcalyphaindicaandTinosporacordifolia in cancer chemotherapy, was tested on EAC cells. The Antitumor efficacy of the herbal based natural products was evaluated with relevant carcinogenesis indicators in Ethno Veterinary Herbal Research and Training Unit for Livestock Health Care, Thanjavur. The Combined extracts of Acalyphaindica and Tinosporacordifoliawas found to have potential anti cancer activity from the in silico and in vitro studies.ArticleItem Open Access An Evaluation of the Anti-Cancerous Effects of Tinospora cordifolia and Aristolochia bracteolate Using CADD (Computer Aided Drug Discovery) Tools(2018) Joseph, Cecilia; Preetha, S.; Sujatha, P.L.; Balachandran, C.; Punniamurthy, N.; TANUVASCancer is an imminent global disaster and the incidence of cancer is widespread in animals with malignant characters and lesions like human beings. Keeping in view the importance and advantages of combination therapy using herbal based natural EVP products on the tumour cells, an attempt to explore the influence of combined effect of Aristolochia bracteolata and Tinospora cordiflora’ was analyzed using in silico approach. It was found that the combined effect of Aristolochia bracteolate and Tinospora cordiflora have shown to possess significant anti-proliferative and anti- apoptotic properties. The active principles from both the plants possess significant pharmaco kinetic and pharmacodynamics effects. As they work synergistically, the combined effect may be a promising drug entity to enter the evidence based therapeutics for cancer.PresentationItem Open Access Genome Microevolution of Prions in Various Species in Comparison with Avian(TANUVAS, Chennai, 2007-08) Aruni, A. Wilson; Sujatha, P.L.; Dhanapalan, P.ArticleItem Open Access Identification of Therapeutic Inhibitors for the Treatment of Oral Cancer using Herbal Bio-Active Principles by Means of Computational Approach(2016) Sujatha, P.L.; Reddy, Ravi; Mahendra, Jaideep; Kumanan, K.; Kumarasamy, P.; Preetha, S.P.; Pavithra, S.; TANUVASOral cancer is a type of cancer which targets mouth and surrounding tissues. It includes, lips, cheeks, tongue, sinuses,throat. Oral cancer could be life threatening, if not diagnosed in early stages. American Cancer Society says that men are at more risk than women. Oral cancer can be treated like any other cancers. But still the efficacy of the drugs is questionable. Hence, an attempt was tried to find the suitable inhibitors for the treatment of oral cancer using bioactive principles of Cissus quadrangularis, targeting SIRT3,P21 and P53. The Resveratol in Cissus quadrangularis has showed very good interaction with two target proteins involved in oral cancer viz., SIRT3 and P21. Resveratol also has very good pharmacodynamic and pharmacokinetic properties. This study concludes that Resveratol in Cissus quadrangularis has the potential to serve as an anticancerous drug. However, the results might be validated through in-vivo and in-vitro studies to prove the efficacy of the active compounds and to protect the mankind from oral cancer.ThesisItem Open Access IN SILICO ANALYSIS OF ACTIVE PRINCIPLES PRESENT IN GYMNEMA SYLVESTRE AGAINST INSULIN RECEPTOR ON ANTI - DIABETIC EFFECT IN TYPE I AND TYPE II DIABETES(2013) Premchand, S.; Kumarasamy, P.; Sujatha, P.L.; Sureshkannan, S.; TANUVASDiabetes is a metabolic disease characterized by hyperglycemia resulting from defects in insulin secretion and its action. Diabetes can be categorized into three types namely type l diabetes, type ll diabetes and gestational diabetes. The chronic hyperglycemia oi‘ diabetes is associated with long-term damage, dysfunction and failure of various organs mainly the eyes, kidneys, nerves, heart and blood vessels. Generally insulin is used in the treatment of diabetes.PresentationItem Open Access In Silico Study on the Interactions of Certain Anti Cancerous Peptides with Cancerous Proteins(TANUVAS, Chennai, 2007-08) Poongodi, A.; Aruni, A. Wilson; Sujatha, P.L.; Gajendran, K.; Dhanapalan, P.ThesisItem Open Access Insilico Analysis of Binding Capacity of Flavanolto Obesity Receptor Molecule by Computational Approach of Small Molecule Protein Docking Method(TANUVAS, Chennai, 2013) Sumathi, S.; Sujatha, P.L.; Sureshkannan, S.; TANUVAS