Designing of Antiviral Sirna for Targeting HIV-1 Subtype C in India
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Date
2014-10
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Abstract
Acquired Immunodeficiency Syndrome (AIDS) is one of the top
deadly diseases in the world. The recent studies confirm that neither
behavioral therapies nor current antiretroviral drugs are likely to cure
HIV infection completely. RNA interference (RNAi) has raised the
possibility of developing a new generation of strategies that can
prevent HIV-1 replication. Since HIV has high mutation rate,
RNAican be made effective only by targeting highly conserved coding
regions with optimal stable siRNAs. This study was mainly focused on
the identification of highly conserved target sites and designing of
stable antiviral siRNAs against HIV-1 subtype C in India. Here, the
mutations in HIV-1 subtype C in India were studied using the tool
HIVseq. The evolutionary pathway of HIV-1 subtype C in India was
inferred through phylogenetic analysis using MEGA4 in the present
study. Based on the mutational and phylogenetic studies, the highly
conserved target sites were identified using BioEdit. Stable, optimal
siRNAs were designed against these conserved target sites using
siRNA selection server at Whitehead Institute. siRNAs designed
against these conserved target sites were anticipated to resist viral
mutational escape and off-target gene silencing. These optimal siRNAs
would increase the durability of RNAi against HIV-1 subtype C.
Description
TNV_BM_5(10)_Oct2014_78-88
Keywords
Veterinary Science, Bioinformatics Centre & ARIS Cell