Browsing by Author "Meignanalakshmi, S."
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ThesisItem Open Access PRODUCTION AND CHARACTERIZATION OF POLY 3-HYDROXYBUTYRATE MICROSPHERES FOR CONTROLLED DRUG RELEASE(TANUVAS, Chennai, 2015) Swornakumari, C.; Meignanalakshmi, S.; Senthilkumar, T.M.A.; TANUVASIn the present study PHB producing Bacillus amyloliquefaciens KT722771 was isolated from slaughter house waste cattle rumen fluid and screened for PHB production by Psudan black staining. PHB production was confirmed by Nile blue, Nile red, Acridine orange.ArticleItem Open Access Production of Cellulase from Bacillus subtilis under Solid- State Fermentation Using Fiber Wastes of Palmyra Palm(Int. J. Curr. Microbiology and Applied Sciences, 2017) Roopa, R.; Charulatha, M.; Meignanalakshmi, S.; TANUVASIn the present study, the production of cellulase has been carried out using novel natural waste as substrates by Bacillus subtilis isolated from slaughter house waste rumen fluid. Bacillus subtilis was screened for cellulase production by Congo red assay. pH and temperature were optimized. Agricultural wastes such as Hay, fiber waste of palmyra palm and banana bracts were used as a substrate for cellulase production. Substrates with or without pretreatment using NaOH solution were used for solid state fermentation. After 48hrs of fermentation, extracts were drawn and tested for enzyme activity by DNSA method and total protein content by Lowry’s method. Cellulase activity was found to be 0.1519, 0.0759 and 0.05557 μmol ml-1min-1 when fibers of palmyra palm, hay and banana bracts were used as substrates. Raw cellulose content was found to be 37%, 32% and 23.2% in fibers of palmyra palm, hay and banana bracts respectively. Fibers of palmyra palm were found to be having maximum enzyme activity of 0.1519μmol ml-1min-1 at pH 7, at 48hrs of incubation.ArticleItem Open Access Rapid detection of Mycobacterium bovis in clinical samples using THP-1 cells(2007) Parthiban, M.; Jaiganesh, R.; Aruni, A. Wilson; Meignanalakshmi, S.; Ramadass, P.; TANUVASThe rapid isolation of M. bovis was attempted in THP-l cells (Macrophage cell line). THP-l cells infected with M. bovis showed characteristic cytopathic effect 48 hr after infection. The bacteria had grown very well in the first passage itself, without any blind passages in cell culture. The presence of bacteria in the cell culture fluid was confirmed by Ziehl-Neelsen staining and nested PCR amplification of insertion sequences.