Rapid detection of Mycobacterium bovis in clinical samples using THP-1 cells
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Date
2007
Journal Title
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Abstract
The rapid isolation of M. bovis was attempted in THP-l cells (Macrophage cell line). THP-l cells infected with M. bovis showed characteristic cytopathic effect 48 hr after infection. The bacteria had grown very well in the first passage itself, without any blind passages in cell culture. The presence of bacteria in the cell culture fluid was confirmed by Ziehl-Neelsen staining and nested PCR amplification of insertion sequences.
Description
TNV_AV_77(3)_2007_265-270
Keywords
Veterinary Science, Animal Biotechnology