Production of Cellulase from Bacillus subtilis under Solid- State Fermentation Using Fiber Wastes of Palmyra Palm
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Int. J. Curr. Microbiology and Applied Sciences
Abstract
In the present study, the production of cellulase has been carried out using novel
natural waste as substrates by Bacillus subtilis isolated from slaughter house waste
rumen fluid. Bacillus subtilis was screened for cellulase production by Congo red
assay. pH and temperature were optimized. Agricultural wastes such as Hay, fiber
waste of palmyra palm and banana bracts were used as a substrate for cellulase
production. Substrates with or without pretreatment using NaOH solution were
used for solid state fermentation. After 48hrs of fermentation, extracts were drawn
and tested for enzyme activity by DNSA method and total protein content by
Lowry’s method. Cellulase activity was found to be 0.1519, 0.0759 and 0.05557
μmol ml-1min-1 when fibers of palmyra palm, hay and banana bracts were used as
substrates. Raw cellulose content was found to be 37%, 32% and 23.2% in fibers
of palmyra palm, hay and banana bracts respectively. Fibers of palmyra palm were
found to be having maximum enzyme activity of 0.1519μmol ml-1min-1 at pH 7, at
48hrs of incubation.
Description
Keywords
Veterinary Science, Animal Biotechnology