Browsing by Author "Latha, B.R."
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ArticleItem Open Access Anthelmintic Activity of Essential Oils of Cymbopogan Nardus and Azadirachta Indica on Fasciola Gigantica(TANUVAS, Chennai, 2010-09) Jeyathilakan, N.; Murali, K.; Anandaraj, A.; Latha, B.R.; Basith, S. Abdul; TANUVASThe efficacy of essential oils of Cymbopogan nardus (citronella) and Azadirachta indica (neem) on Fasciola gigantica was assessed in vitro. The essential oil of citronella showed a potential anthelmintic activity whereas neem oil did not show any significant effect. Study revealed that 1% citronella oil showed anthelmintic effect on live Fasciola gigantica. The in vitro anthelmintic effect was evaluated in comparison with control and score index was made based on gross motility. The morphological variation and histopathological changes in treated and control fluke was studied.ArticleItem Open Access Assessment of cell mediated immune response in rabbits immunized with affinity purified 35 kDa midgut antigen of Rhipicephalus haemaphysaloides ticks(Journal Impact Factor, 2015-01) Kavitha, K.T.; Latha, B.R.; Raj, G. Dhinakar; TANUVASCell mediated immune response to immunoaffinity chromatography purified midgut antigen of Rhipicephalus haemaphysaloides ticks in rabbits was studied by using lymphocyte transformation test. This test was carried out by using 5-bromo-20-deoxy-uridine kit method. The blastogenic response of peripheral blood lymphocytes of normal rabbit to different concentrations of antigen and mitogen (Con A) showed that 2 lg of antigen and 2 lg of mitogen gave maximum stimulation index. The antigen specific responsiveness of immunized rabbits with affinity purified 35 kDa midgut antigen was highly significant (P B 0.01) compared to mitogen. The maximum lymphocyte stimulation index (LSI) of 2.47 was observed on 49 day post immunization in immunized group. The lymphocytes separated from control animal cultured in RPMI1640 medium with 2 lg of antigen and 2 lg of mitogen (Con A) were never stimulated and their LSI values were below 2.0.ArticleItem Open Access Cestodiasis in a Blue Rock Pigeon (Columbia livia)(2015-04) Ramesh, S.; Latha, B.R.; Kavitha, K.T.; Vijayarani, K.; Palanivelrajan, M.; Senthilkumar, K.; Jayathangaraj, M.G.; TANUVASThe carcass of a Blue Rock Pigeon revealed haemorrhages in the entire digestive tract with tape worm segments, confirned to be Cotugnia sp in the lumen of the intestine. lntestinal contents also revealed the presence of eggs of Cotugnia sp egggs on microscopic examination.ArticleItem Open Access Clinicopathological Findings and Successful Management of Concurrent Infection of Babesiosis and Ehrlichiosis In Dogs(Indian Vet. J., 2013-08) Vijayalakshmi, P.; Srinivasan, S.R.; Vairamuthu, S.; Mangalagowri, A.; Latha, B.R.; Nambi, A.P.; TANUVASThe study was carried out on 12 dogs that were diagnosed for concurrent infection of ehrlichiosis and babesiosis. Varied clinical manifestations including lethargy, fever, lymphadenomegaly and epistaxis were recorded. Ticks recovered from the affected dogs were identi ed as Rhipicephalus sanguineus. Haematology revealed marked anaemia, thrombocytopenia, lymphopenia, monocytosis and anisocytosis. Hypoalbuminemia and increase SAP levels were indicative of moderate hepatopathy. Combination of parenteral diminazene aceturate and oral doxycycline with necessary supportive was found to be highly effective in the treatment of concurrent infection of ehrlichiosis and babesiosis in dogs.ArticleItem Open Access Concurrent Marek's Disease and Cestodiasis in a Blue Rock Pigeon (Columbia Livia)(Indian Veterinary Journal, 2015-05) Ramesh, S.; Balachandran, C.; Vijayarani, K.; Latha, B.R.; Kavitha, K.T.; Palanivelrajan, M.; Senthilkumar, K.; Savithri, M.; Pazhanivel, N.; Jayathangaraj, M.G.; TANUVASA Blue Rock pigeon (Columbia livia) found dead in Chennai revealed congestion of the serosal surface of the entire digestive tract and haemorrhages in the mucosal surface with tape worm segments, identified as Cotugnia sp in the lumen of the intestine. Histopathological examination of the liver and kidney as well as PCR confirmed Marek's disease.ArticleItem Open Access Depluming Itch in a Parrot(INTAS Polivet, 2013) Kavitha, S.; Yamini, S. Hamsa; Nagarajan, B.; Latha, B.R.; Nambi, A.P.; TANUVASCnemidocoptes dermatitis was recorded in a four month old parrot and successful management using fibronil spot on.OtherItem Open Access Deworming of Poultry(TANUVAS, 2012) Soundararajan, C; Raman, M.; Mathialagan, P.; Latha, B.R.; Sundar, S.T. Bino; Jeyathilakan, N.; Gomathinayagam, S.; Thenmozhi, V.; Aravindan, K.; Karthik, M.; Basith, S. AbdulOtherItem Open Access Diagnosis and Management of Choriodiscoides Caviae Infestation in Dunkin Hartley Guinea Pigs(TANUVAS, 2016-02) Gayathri, K.; Ramesh, S.; Vijay, K.; Latha, B.R.; Hemalatha, S.ArticleItem Open Access Diagnosis of cystic echinococcosis in buffaloes by native 8 kDa antigen using latex agglutination test (LAT)(Springer Verlag, 2016-10) Sheeba, A.; Sangaran, A.; Latha, B.R.; TANUVASThe dog tapeworm Echinococcus granulosus is the causative agent of cystic hydatid disease in domestic/ wild herbivores animals and man. Accurate immunodiagnosis of the infection requires highly specific and sensitive antigens. The aim of this study was to develop and evaluate immunoassays with principles of precipitation, agglutination for the identification of buffaloes infected with hydatid cyst which would allow the monitoring of animals from endemic areas and identifying infected animals prior to slaughter. The immunoassays were developed and validated using hydatid specific, non-cross reactive low molecular weight 8 kDa hydatid cyst fluid protein. Sera used for the assay validations were obtained from 200 buffaloes infected naturally with hydatid cyst and 200 noninfected buffaloes. The diagnostic sensitivity with latex agglutination test was 98.67 %. It should be useful for the conformation of hydatid cyst infected individual sheep.OtherItem Open Access Dot Eia in Detection of Circulating Antigen in Sheep Hydatidosis(TANUVAS, 2016-02) Sangaran, A.; Sheeba, A.; Sundar, S.T. Bino; Latha, B.R.ArticleItem Open Access Factors influencing on prevalence of Cryptosporidium infection in south Indian dairy calves(J Parasit Dis, 2012-08) Venu, R; Latha, B.R.; Basith, S. Abdul; Sreekumar, C; Raj, G. Dhinakar; Raman, M; TANUVASArticleItem Open Access Factors influencing on prevalence of Cryptosporidium infection in south Indian dairy calves(Journal Impact Factor, 2013-07) Venu, R.; Latha, B.R.; Basith, S. Abdul; Sreekumar, C.; Raj, G. Dhinakar; Raman, M.; TANUVASThe objectives of the present study were to report the influence of factors like age, sex, breed, dung consistency and rearing system on prevalence of Cryptosporidium spp. in south Indian cattle. Two-step nested PCR was employed for detection of Cryptosporidium infection in dairy calves of south Indian states viz., Andhra Pradesh, Karnataka, Kerala, Tamil Nadu and union territory i.e., Puducherry. A total of 459 dung samples from the calves were subjected to nested PCR, 182 were found positive with prevalence percent of 39.65. Age wise comparison showed a high prevalence of Cryptosporidium in the age group of one month old calves. This concludes that the cryptosporidiosis is highly age dependent with young calves showed the highest prevalence. Depending on the group had consistency of dung, the highest prevalence of Cryptosporidium was observed in semi-solid dung, followed by formed and the diarrhoeic group animals. Female calves showed slightly higher prevalence rate than male animals. Cow calves had an overall prevalence percent of 40.75 and the infection rate in buffalo calves was 36.28 %. In relation to rearing system, individual animals had 42.18 % and farm animals showed 38.46 % of Cryptosporidium infection. In conclusion, the prevalence of Cryptosporidium in dairy calves should be correlated with the factors like age, sex, breed, dung consistency and rearing system of the animal to arrive at a reliable epidemiological data on bovine cryptosporidiosis.ArticleItem Open Access Genotypic Characterization of Buffalo Isolates of Echinococcus granulosus(2015-05) Sheeba, A.; Sangaran, A.; Latha, B.R.; Raja, A.; TANUVASCystic echinococcosis (CE) caused by the larval stage of the cestode Echinococcus granulosus is one of the most widespread zoonoses of veterinary and medical importance. Molecular techniques have allowed the identification of 10 different genotypes (G1-G10)of the parasite. Numerous studies have provided evidence that Echinococcus granulosus exists as a complex of different strains, that differ in wide variety of criteria that have impact on the epidemiology, pathology and control of cystic hydatid disease (CHD). On sequence analysis of mitochondrial COX1 and NADH genes showed the occurrence of the G1 genotype, the common sheep strain, G2 genotype, the Tasmanian sheep strain, the G3 genotype, the buffalo strain. The present study is an update of E.granulosus strains by genotyping a large samples of isolates and checking out genetic differentiation within and among the G1,G2,G3 genotype using additional gene marker, the RRNS gene. Sequencing of RRNS gene revealed fixed nucleotide substitution among G1,G2 and G3 genotypes. This study provides further evidence of the occurrence of the E.granulosus G1 and G2 sheep strain.ArticleItem Open Access Hydatid Cyst in Liver - A Case Report(2014-07) Sangaran, A.; Sheeba, A.; Sundar, S.T. Bino; Latha, B.R.; TANUVASHydatid disease (Cystic echinococcosis) is a parasitic disease that remains a clinical problem worldwide, especially in areas where animal husbandry and subsistence farming form an integral part of community. In humans, accidental infection occurs as a result of ingestion of eggs of the dog tapeworm Echinococcus granulosus along with contaminated food and water. Among the visceral organs affected in human beings, liver is the most commonly involved organ. We present a case report of a 23 year old female patient who presented initially with abdominal pain with loss of appetite and the problem was persisting for nearly five years. For confirmation of hydatid cyst, the patient was referred to the Department of Veterinary Parasitology for serological confirmation. Using the sera of the suspected patient, Latex agglutination test (LAT) was performed and the sample was found positive.ArticleItem Open Access Hydatid Cyst In Liver - A Case Report in Human(2015-04) Sheeba, A.; Sangaran, A.; Latha, B.R.; TANUVASHydatid disease (Cystic echinococcosis) is a parasitic disease that remains a clinical problem worldwide, especially in areas where animal husbandry and subsistence farming form an integral part of community. In humans, accidental infection occurs as a result of ingestion of eggs of the dog tapeworm Echinococcusgranulosus along with contaminated food and water. Among the visceral organs affected in human beings, liver is the most commonly involved organ. We present a case report of a 23 year old female patient who presented initially with abdominal pain with loss of appetite and the problem was persisting for nearly five years. For confirmation of hydatid cyst, the patient was referred to the Department of Veterinary Parasitology for serological confirmation. Using the sera of the suspected patient, Latex agglutination test (LAT) was performed and the sample was found positive.ArticleItem Open Access In vitro Evaluation of Synthetic Pyrethroid Resistance in Rhipicephalus Sanguineus Ticks of Chennai(Journal of Veterinary Parasitology, 2011) Mathivathani, C.; Basith, S. Abdul; Latha, B.R.; Raj, G. Dhinakar; TANUVASEngorged female Rhipicephalus sanguineus ticks were collected from dogs with a previous history of acaricidal treatment with synthetic pyrethroids (SP) from small animal clinics, Madras Veterinary College, Chennai. The collected ticks were subjected to adult immersion test (AIT) with discriminating dose of 0.075 g per litre of deltamethrin and flumethrin as recommended by FAO (1999). Three trials were conducted using 90 ticks. The results of AIT found that the ticks collected at random from dogs were resistant to SP. Deltamethrin and flumethrin treated ticks revealed an average 64.72 and 59.35 per cent resistance, respectively.ArticleItem Open Access In Vivo Immunomodulatory Effects of Ixidid Ticks on Ovine Circulating T- and B-Lymphocytes(Parasite Immunology, 2004) Dharmendra, K.V. Boppana; Raj, G. Dhinakar; John, Lalitha; Stephen, K. Wikel; Latha, B.R.; Gomathinayagam, S.; TANUVASSelected aspects of the ovine immune system were examine during the course of repeated infestations with the ixodid ticks, Haemaphysalis bispinosa and Hyalomma anatolicum anatolicum that naturally infest sheep, either individually or together. By the use of flow cytometry it was shown that total T-lymphocyte numbers were significantly reduced from the sixth through the ninth days of all infestations. Gammaldelta (y8^+) and CD8^+ T-lymphocytes were significantly depleted during tick feeding in all infested groups. CD4^+ T-lymphocyte levels were significantly increased during seconday H. Bispinosa and mixed species infestations. Hyalomma anatolicum anatolicum caused a significant increase in ciruculating B-lymphocytes over several days in both initial and seconday infestations. All infested sheep had increased CD4|CD8 and decreased T|B lymphocyte ratios during exposure to both ticks. Bromodeoxyuridine (BrdU) ELISA was used to measure in vitro proliferation of peripheral blood mononuclear cells stimulated with the T-lymphocyte mitogen Concanavalin A (Con A) after their collection from infested sheep. Significant suppression of in vitro proliferation occurred during first and secondary infestations with H. Bispinosa, H. a. Anatolicum and with both tick species together, beginning on the sixth day of infestation in all cases. These important tick species of sheep significantly modulate the numbers of immune effector cells and proliferation of T-lymphocytes derived from infested animals.ArticleItem Open Access Kalicephalus sp. in a captive Russell’s viper: a case report(Journal Impact Factor, 2014-07) Kavitha, K.T.; Latha, B.R.; Sundar, S. T. Bino; Jayathangaraj, M. G.; Kumar, K. Senthil; Sridhar, R.; Basith, S. Abdul; TANUVASThe occurrence of Kalicephalus sp. of hookworms in a Russell’s viper snake maintained in Chennai snake park trust, Chennai is reported. These worms were found in the stomach and intestine. They were cylindrical, thread like and had an elongate body. Anterior end was obliquely truncated with bivalvular buccal capsule. Short, thick and muscularised oesophagus ending in a rounded bulb was observed. In males, a well developed trilobed bursa was observed and the spicules were short and equal. Typical strongyle type eggs were found in the uterus of female worms as well as in the intestinal contents.
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