Assessment of cell mediated immune response in rabbits immunized with affinity purified 35 kDa midgut antigen of Rhipicephalus haemaphysaloides ticks
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Date
2015-01
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Abstract
Cell mediated immune response to immunoaffinity
chromatography purified midgut antigen of Rhipicephalus
haemaphysaloides ticks in rabbits was studied by using
lymphocyte transformation test. This test was carried out by
using 5-bromo-20-deoxy-uridine kit method. The blastogenic
response of peripheral blood lymphocytes of normal rabbit to
different concentrations of antigen and mitogen (Con A)
showed that 2 lg of antigen and 2 lg of mitogen gave maximum
stimulation index. The antigen specific responsiveness
of immunized rabbits with affinity purified 35 kDa midgut
antigen was highly significant (P B 0.01) compared to
mitogen. The maximum lymphocyte stimulation index (LSI)
of 2.47 was observed on 49 day post immunization in
immunized group. The lymphocytes separated from control
animal cultured in RPMI1640 medium with 2 lg of antigen
and 2 lg of mitogen (Con A) were never stimulated and their
LSI values were below 2.0.
Description
TNV_JPD_2015_39(1)90-93
Keywords
Veterinary Science