Assessment of cell mediated immune response in rabbits immunized with affinity purified 35 kDa midgut antigen of Rhipicephalus haemaphysaloides ticks

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dc.contributor.authorKavitha, K.T.
dc.contributor.authorLatha, B.R.
dc.contributor.authorRaj, G. Dhinakar
dc.contributor.authorTANUVAS
dc.date.accessioned2018-04-09T11:25:42Z
dc.date.available2018-04-09T11:25:42Z
dc.date.issued2015-01
dc.descriptionTNV_JPD_2015_39(1)90-93en_US
dc.description.abstractCell mediated immune response to immunoaffinity chromatography purified midgut antigen of Rhipicephalus haemaphysaloides ticks in rabbits was studied by using lymphocyte transformation test. This test was carried out by using 5-bromo-20-deoxy-uridine kit method. The blastogenic response of peripheral blood lymphocytes of normal rabbit to different concentrations of antigen and mitogen (Con A) showed that 2 lg of antigen and 2 lg of mitogen gave maximum stimulation index. The antigen specific responsiveness of immunized rabbits with affinity purified 35 kDa midgut antigen was highly significant (P B 0.01) compared to mitogen. The maximum lymphocyte stimulation index (LSI) of 2.47 was observed on 49 day post immunization in immunized group. The lymphocytes separated from control animal cultured in RPMI1640 medium with 2 lg of antigen and 2 lg of mitogen (Con A) were never stimulated and their LSI values were below 2.0.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810043093
dc.keywordsRhipicephalus haemaphysaloides - Lymphocyte stimulation index and affinity chromatography - Midgut antigenen_US
dc.language.isoenen_US
dc.pages90-93en_US
dc.publisherJournal Impact‎ Factoren_US
dc.relation.ispartofseries;1
dc.subjectVeterinary Scienceen_US
dc.titleAssessment of cell mediated immune response in rabbits immunized with affinity purified 35 kDa midgut antigen of Rhipicephalus haemaphysaloides ticksen_US
dc.title.alternativeJournal of Parasitic Diseasesen_US
dc.typeArticleen_US
dc.volume39en_US
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