Diagnosis of cystic echinococcosis in buffaloes by native 8 kDa antigen using latex agglutination test (LAT)
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Date
2016-10
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Verlag
Abstract
The dog tapeworm Echinococcus granulosus is
the causative agent of cystic hydatid disease in domestic/
wild herbivores animals and man. Accurate immunodiagnosis
of the infection requires highly specific and sensitive
antigens. The aim of this study was to develop and evaluate
immunoassays with principles of precipitation, agglutination
for the identification of buffaloes infected with hydatid
cyst which would allow the monitoring of animals from
endemic areas and identifying infected animals prior to
slaughter. The immunoassays were developed and validated
using hydatid specific, non-cross reactive low
molecular weight 8 kDa hydatid cyst fluid protein. Sera
used for the assay validations were obtained from 200
buffaloes infected naturally with hydatid cyst and 200 noninfected
buffaloes. The diagnostic sensitivity with latex
agglutination test was 98.67 %. It should be useful for the
conformation of hydatid cyst infected individual sheep.
Description
TNV_JPD_2016_40(4)1401-1405
Keywords
Veterinary Science