Browsing by Author "Kumar, O.R. Vinodh"
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ArticleItem Open Access Comparison of Diagnostic tests for Paratuberculosis(Indian Veterinary Journal, 2012-06) Kumar, O.R. Vinodh; Faris, D.; Senthil, N.R.; Gunaseelan, L.; TANUVASSixty three paratuberculosis suspected dairy animals were taken for this study and subjected to single intradermal test, faecal culture and IS900 nested PCR. Thirty three animals were found positive by single intradermal test, 40 samples were positive by faecal culture and 58 samples were positive by nested PCR. 40 faecal samples out of 63 were positive by IS900 primary PCR assay for Mycobacterium avium subsp. Paratuberculosis (Map) and 23 faecal samples, which gave negative results in the primary PCR, were subjected to secondary PCR assay. Of the 23 samples, 18 samples gave positive results in the IS900 nested PCR (nPCR), giving better sensitivity than single amplification PCR. The chi-square analysis showed a highly significant difference between the tests (P<0.01). The percentage positivity of single intradermal test, faecal culture and nested PCR assay in this study was 52.38% (33/63), 63.49% (40/63) and 92.06% (58/63), respectively. This study suggests that IS900 nested PCR could be used as a potential diagnostic tool in animals suspected of paratuberculosis.ThesisItem Open Access Comparison of Single Intradermal Test and Polymerase Chain Reaction for the Diagnosis of Bovine Paratuberculosis in Organized Farms(TANUVAS, 2003) Kumar, O.R. Vinodh; TANUVAS; Ganesan, P.I.; Nedunchelliyan, S.; Raj, G. DhinakarArticleItem Open Access Influence of Functional Feeds on Egg Production, Egg Quality and Serum Lipid Profile(Indian Veterinary Journal, 2012-09) Sujatha, T.; Kumar, O.R. Vinodh; Prabakaran, R.; TANUVASAn experiment with functional feeds rich in omega-3 fatty acids, carotenoid pigments, Vitamin-E and selenium was carried out to enrich the nutritional value of chicken eggs. The study revealed that dietary manipulation did not affect egg production and egg quality adversely. Moreover, the hens which consumed the functional had a favourable lipid profile for optimal health.ArticleItem Restricted Milk as a Potential Source of Mycobacterium avium Subsp Paratuberculoosis Infection(Indian Veterinary Journal, 2012-07) Kumar, O.R. Vinodh; Gunaseelan, L.; TANUVASAnimals with clinical and subclinical infections of Johne's disease shed Mycobacterium avium subsp paratuberculosis (MAP) bacteria in both milk and feces. Contamination of milk by MAP is widely reported in cattle. Milk is a potential source of disease transmission in young animals and milk has also been implicated as a potential source of MAP for humans since viable organisms have been recovered from commercial pasteurized milk (Grant et al., 2002). MAP has been isolated from confirmed cases of Crohn's disease in humans, indicating its zoonotic potential (Singh et al., 2008).ThesisItem Open Access Molecular Modelling and Interactions of Human Brk (PTK6) Protein Tyrosine Kinase(TANUVAS, 2005) Kumar, O.R. Vinodh; TANUVAS; Thulasi, G.; Raj, G. Dhinakar; Sujatha, P.L.ArticleItem Open Access Paratuberculosis in Young Calves(Indian Veterinary Journal, 2013-01) Kumar, O.R. Vinodh; Senthil, N.R.; Faris, D.; TANUVASMycobacterium avium subsp. paratuberculosis (MAP), a slow-growing acid-fast bacterium, causes Johne's disease or paratuberculosis, a progressive granulomatous enteritis of ruminants, characterized by chronic or intermittent, therapy-resistant diarrhea, severe emaciation and eventual death. Cattle become infected as young calves when suckling teats get contaminated with MAP infected faeces. MAP infection often occurs through faecal-oral route, due to ingestion of contaminated faecal material. Calves less than 30 days of age are most susceptible to infection and most cattle are infected before they were four months of age (Merkal et. al., 1987). This study was taken to know the prevalence of MAP infection in calves below 6 months of age.ArticleItem Open Access A PRELIMINARY STUDY ON THE USEFULNESS OF ELISA AND GAMMA INTERFERON ASSAY IN BUFFALO AND WHITE CATTLE PARATUBERCULOSIS DIAGNOSIS(International Buffalo Information Center, 2010-12) Kumar, O.R. Vinodh; Gunaseelan, L.; Rani, K. Vijaya; Ronald, B.S.M.; TANUVASThirty-seven animals aged over 2 years in a paratuberculosis endemic herd were selected to study the usefulness of ELISA and gamma interferon assay in bovine paratuberculosis diagnosis. All the animals were screened by a comparative single intradermal test for tuberculosis and paratuberculosis. Of the 37 animals tested by gamma interferon assay and ELISA, 21 animals were positive by γ- IFN and nine animals were positive by ELISA.ArticleItem Open Access Prevalence of Bovine Paratuberculosis in Sur-urban Chennai City(Indian Veterinary Journal, 2013-01) Kumar, O.R. Vinodh; Senthil, N.R.; Faris, D.; TANUVASParatuberculosis. a chronic granulomatous bowel disease of ruminants is caused by Mycobacterium avium subsp. Paratuberculosis. Animals infected early in life but often do not develop clinical disease until 3 years of age (Chiodini et. al., 1984). This study was conducted on the slaughterhouse prevalence of bovine paratuberculosis in the city of Chennai.ArticleItem Open Access Seroprevalence of Equine Herpes Virus in Tamilnadu by ELISA(Indian Veterinary Journal, 2012-09) Senthil, N.R.; Kumar, O.R. Vinodh; Faris, D.; TANUVASEquine Herpesvirus has been identified to exist as EHV-1, EHV-3 and EHV-4 which are all members of the subfamily Alphaherpesvirane. EHV-2 and EHV-5 are members of subfamily Gammaherpesvirinae (Crabb and Studdert, 1993). EHV-2 is the most common viral cause of abortion in mares. In India, the association of EHV-1 with nervous disease (Shankar and Yadav, 1986) and respiratory disease (Uppal et al., 1991) in equines have been documented. The following study was undertaken to assess the seroprevalence of Equine Herpes Virus (EHV-1) in horses, donkeys and mules in some parts of Tamil Nadu.ArticleItem Open Access Status of Bovine Paratuberculosis in Organized Farms of Tamilnadu(Indian Veterinary Journal, 2011-11) Kumar, O.R. Vinodh; Ganesan, P.I.; TANUVASThe present study was undertaken to assess the status of bovine Johne's disease in organized farms in Tamil Nadu.