STUDY ON GENETIC DIVERSITY IN DIFFERENT STOCKS OF AMUR COMMON CARP (CYPRINUS CARPIO VAR. HAEMATOPTERUS) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS
Loading...
Date
2022
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
Three stocks of Amur common carp in different geographical locations viz. Tamil Nadu stock (Krishnagiri hatchery), Karnataka stock (Hesaraghatta) and Kolkata stock (Das fish hatchery) representing different environmental conditions were collected at selected breeding centers. The three stocks were acclimatized and reared in laboratory conditions with similar environmental and management conditions. RAPD -PCR analysis was performed for different stocks of Amur common carp. Of the 140 decamer primers screened, eight primers (OPA- 04, OPA-06, OPA-10, OPA- 20, OPB-17, OPF- 09, OPF- 10, and OPG- 9) which has given consistent and more polymorphism were selected for RAPD – PCR analysis. RAPD – PCR analysis shows that the Tamil Nadu stock shows higher polymorphism (82.38%) in comparison with the other two stocks. The higher Nei’s heterozygosity (0.2395) was observed in Tamil Nadu stock. The highest Shannon’s diversity (0.3079) was observed in Tamil Nadu stock. In terms of genetic identity and genetic distance, Kolkata and Karnataka stocks showed a maximum genetic distance of 0.5075 and a minimum Genetic identity of 0.6020. while the Kolkata and Tamil Nadu stock showed a maximum genetic identity of 0.7234 and a minimum genetic distance of 0.3238. The study also shows that Tamil Nadu stock is closely related to Kolkata stock and distantly related to Karnataka stock. Growth trials indicated better performance of Tamil Nadu stock in comparison with other stocks. The r2 value of (0.5710) between the percentage of polymorphism and mean body weight attained for different stocks of Amur common carp shows that the percentage of polymorphism and mean body weight attained are Moderate Positively correlated.