A STUDY ON THE ANTIOXIDANT ACTIONS OF FISH OIL & VIT. E + SELENIUM IN BROILERS
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Date
2006
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT:
The antioxldant potentials of fish oil and vltamin E + selenlum was
assessed for prophylactic and therapeutic management of free radical
induced damage In an experimental model of oxidative stress induced by an
ionophorous coccldiostat, maduramicin, at a toxic level of 8 ppm in feed
A total of one hundred and twenty, male broiler chicks (Cobb strain) of
day old age were randomly divided into eight groups, consisting of fifteen In
each grol;p. Groups 1, 3 and 4 were maintained as basal diet control, f~sho il
control and vlt E + Se control, respectively. Group 2 was maintarned with
madurarnic~n @ 8 ppm in feed throughout for 6 wks and was maintained as
toxic control wlthout any treatment. Groups 5 and 6 were fed w~thfi sh oil @
2% in feed and vlt E (300 mglkg) + Se (0.3 mglkg). respecttvely along with
madurarn~cln @ 8 pprn in feed throughout 6 wks of the experiment Groups 7
and 8 were fed wlth maduramlcin @ 8 pprn In feed for the first 4 wks (28 days)
of study and thereafter, group 7 was given a fish oil supplemented feed @ 2%
in feed and group 8 with vit E (300 mg/kg) + Se (0.3 mg/kg) In feed till the
termination of the experiment at the end of 6Ih wk. Antioxidant defence profile (GSH-Px, GSH-R, catalase GSH, and
TBARS), b~ornarkers of hepatic damage (AST), lipid profile (total cholesterol.
triglycerides. HDL cholesterol and LDL cholesterol), prote~n profile (total
protein, albumin, globulin and A/G ratio) and immune status (HI titre) were
estimated at the end of 4" wk and 61h wk, except TBARS activity and HI titres
'that were estimated once, at the end of the 61h wk. Histopathological studies
on liver, bursa, heart and skeletal muscle were conducted at the end of the 6'h
wk. Antioxidant enzyme levels and biochemical parameters were significantly altered, and the histopathoiogical studies revealed separation of muscle fibre bundles In the myocardium and areas of necrosis in the liver in
the maduramicin toxic control. These parameters were normal in the controls
(groups 1.3 and 4), and other groups that were given fish oil and vit E + Se
either prophylactically or therapeutically (groups 5, 6, 7 and 8). Thus, it is concluded that fish oil and vit E + Se were effective as ant~oxidants in preventing and countering oxidative stress by facilitating
restoration of antioxidant defence mechanism. Hence, their supplementation
would reduce the ~ncidence of economic losses due to stress. Beneficial
effect of fish oil may be attributed to the presence of omega-3 fatty acids.
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