STUDIES ON IN VITRO ROOTING AND HARDENING OF GISELA 5 (PRUNUS CERASUS X PRUNUS CANESCENS) CHERRY ROOTSTOCK

Simple item page
dc.contributor.advisorTHAKUR, MANISHA
dc.contributor.authorAKHIL, KUMAR
dc.date.accessioned2017-09-07T08:01:53Z
dc.date.available2017-09-07T08:01:53Z
dc.date.issued2017
dc.description.abstractABSTRACT A technique for in vitro rooting and hardening of Gisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock has been developed. In vitro shoot cultures maintained in the department by routine subculturing were used as source of microshoots for rooting experiments. In single step rooting procedure, maximum rooting (100%) was achieved on full strength MS medium fortified with 0.5 mg/l IBA in which thin, long roots without callus were obtained. In two step procedure of rooting, maximum rooting (60.00%) was observed after 24 hours dark incubation in half strength MS broth fortified with 0.1 and 0.4 mg/l IBA followed by transfer to semisolid half strength MS basal medium. At all concentrations of NAA and IAA tested in full strength and half strength MS medium, rooting percentage was low. Thick, long branched roots were formed when IAA was supplemented in full as well as half strength MS medium, whereas, in case of NAA thick, short roots without callus were formed. Addition of activated charcoal (0.4%) in half strength MS medium fortified with 0.5 mg/l IBA showed 40.06% rooting without callus, whereas in phologlucinol (0.1%) supplemented medium only callus was observed at the bases of the shoots which did not lead to root formation. The in vitro rooted plantlets were successfully hardened in sand: soil: FYM (1:1:1) with 100 per cent survival in the month of August. After two months these plants were transferred to bigger pots containing sand: soil: FYM (1:1:1) and are showing normal growth without any morphological variations.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810030683
dc.keywordsGisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock,in vitro rooting and hardening,techniquesen_US
dc.language.isoenen_US
dc.pages38+IVen_US
dc.publisherUHF,NAUNIen_US
dc.subBiotechnologyen_US
dc.subjectnullen_US
dc.themeGisela 5 (Prunus cerasus x Prunus canescens) cherry rootstock,in vitro rooting and hardening,techniquesen_US
dc.these.typeM.Scen_US
dc.titleSTUDIES ON IN VITRO ROOTING AND HARDENING OF GISELA 5 (PRUNUS CERASUS X PRUNUS CANESCENS) CHERRY ROOTSTOCKen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Akhil Final thesis M.Sc. PDF.pdf
Size:
2.03 MB
Format:
Adobe Portable Document Format
Description:
BIOTECHNOLOGY
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses