STUDIES ON IN VITRO ROOTING AND HARDENING OF GISELA 5 (PRUNUS CERASUS X PRUNUS CANESCENS) CHERRY ROOTSTOCK
Abstract
ABSTRACT
A technique for in vitro rooting and hardening of Gisela 5 (Prunus cerasus x Prunus
canescens) cherry rootstock has been developed. In vitro shoot cultures maintained in the
department by routine subculturing were used as source of microshoots for rooting experiments.
In single step rooting procedure, maximum rooting (100%) was achieved on full strength MS
medium fortified with 0.5 mg/l IBA in which thin, long roots without callus were obtained. In two
step procedure of rooting, maximum rooting (60.00%) was observed after 24 hours dark
incubation in half strength MS broth fortified with 0.1 and 0.4 mg/l IBA followed by transfer to
semisolid half strength MS basal medium. At all concentrations of NAA and IAA tested in full
strength and half strength MS medium, rooting percentage was low. Thick, long branched roots
were formed when IAA was supplemented in full as well as half strength MS medium, whereas,
in case of NAA thick, short roots without callus were formed. Addition of activated charcoal
(0.4%) in half strength MS medium fortified with 0.5 mg/l IBA showed 40.06% rooting without
callus, whereas in phologlucinol (0.1%) supplemented medium only callus was observed at the
bases of the shoots which did not lead to root formation. The in vitro rooted plantlets were
successfully hardened in sand: soil: FYM (1:1:1) with 100 per cent survival in the month of
August. After two months these plants were transferred to bigger pots containing sand: soil: FYM
(1:1:1) and are showing normal growth without any morphological variations.
Description
Keywords
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