ISOLATION AND DETECTION OF PATHOGENIC LEPTOSPIRA SPECIFIC NUCLEIC ACID SEQUENCES BY POLYMERASE CHAIN REACTION

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dc.contributor.authorEazhisai, R
dc.contributor.authorRavikumar, G
dc.contributor.authorSenthilkumar, TMA
dc.contributor.authorManimaran, K
dc.contributor.authorTANUVAS
dc.date.accessioned2020-07-23T07:01:19Z
dc.date.available2020-07-23T07:01:19Z
dc.date.issued2018
dc.descriptionTNV_TH_MVM 16043_2018en_US
dc.description.abstractLeptospirosis is a globally emerging zoonotic disease that has significant impact on animal and human health. A study was carried out to isolate and detect pathogenic Leptospira specific nucleic acid sequence by polymerase chain reaction. Out of 133 samples collected from various animals, two isolates from rat urine sample was obtained. The overall percentage of isolation of this study was low as 1.5, whereas the percentage of isolation in rats were 12.5. The genotypic characterisation of the two rat isolates and isolates of dog, cow and human from the Zoonoses Research Laboratory revealed amplification in G1/G2 (285 bp), LipL32 (756 bp), LipL21 (561 bp) and Loa22 (257 bp), which indicated that all the isolates belonged to pathogenic type. Also, the positioning of isolates in the pathogenic clade was further analysed by gene sequencing with partial rpoB gene and confirmed that all the isolates were pathogenic type.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810149681
dc.keywordsIsolation, pathogenic leptospira specific nucleic acid, polymerase chain reactionen_US
dc.language.isoenen_US
dc.pages1-4en_US
dc.subVeterinary Microbiologyen_US
dc.subjectVeterinary Scienceen_US
dc.themeVeterinary Microbiologyen_US
dc.these.typeM.V.Sc.en_US
dc.titleISOLATION AND DETECTION OF PATHOGENIC LEPTOSPIRA SPECIFIC NUCLEIC ACID SEQUENCES BY POLYMERASE CHAIN REACTIONen_US
dc.typeThesisen_US
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