ISOLATION AND DETECTION OF PATHOGENIC LEPTOSPIRA SPECIFIC NUCLEIC ACID SEQUENCES BY POLYMERASE CHAIN REACTION
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Date
2018
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Abstract
Leptospirosis is a globally emerging zoonotic disease that has significant impact on animal and human health. A study was carried out to isolate and detect pathogenic Leptospira specific nucleic acid sequence by polymerase chain reaction. Out of 133 samples collected from various animals, two isolates from rat urine sample was obtained. The overall percentage of isolation of this study was low as 1.5, whereas the percentage of isolation in rats were 12.5. The genotypic characterisation of the two rat isolates and isolates of dog, cow and human from the Zoonoses Research Laboratory revealed amplification in G1/G2 (285 bp), LipL32 (756 bp), LipL21 (561 bp) and Loa22 (257 bp), which indicated that all the isolates belonged to pathogenic type. Also, the positioning of isolates in the pathogenic clade was further analysed by gene sequencing with partial rpoB gene and confirmed that all the isolates were pathogenic type.
Description
TNV_TH_MVM 16043_2018
Keywords
Veterinary Science