Development of micropropagation protocols in Cappris decidua (forsk) edgew

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dc.contributor.advisorKharb, Pushpa
dc.contributor.authorChahar, Om Parkash
dc.date.accessioned2016-11-30T09:48:38Z
dc.date.available2016-11-30T09:48:38Z
dc.date.issued2005
dc.description.abstractIn vitro clonal propagation of Capparis decidua was achieved using nodal part and shoot tip explants from mature trees grown in CCS Haryana Agricultural University, Hisar Campus. Response for shoot regeneration was observed within five days on all the media tested from nodal and shoot tip explants. Shoot regeneration was observed only on MS medium supplemented with 25.0 mg/L adenine sulphate and 5.0 mg/L BAP. Nodal explants gave best shoot regeneration (96%) on the same MS medium supplemented with 25.0 mg/L adenine sulphate and 5.0 mg/L BAP. On sub-culturign the explants on the same medium 10-11 shoots/explant were observed in 95% of explants cultured. Regenerated shoots also subcultured on MS basal + culture supernatant of Acetobacter (4 ml/L) and culture supernatant of Azotobacter 4 ml/L medium formed multiple shoots in 82% of the cases with 14-16 shoots per explant. These shoots were treated with liquid MS basal + 25.0 mg/L IBA for 6 days and then transferred to rooting media. Best rooting was observed 37% on MS basal + 1.0 mg/L IBA and 1.0 mg/L IAA with healthy and strong rooting. These shoots with roots were kept overnight for hardening and then transferred to pots.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/88301
dc.language.isoenen_US
dc.publisherCCSHAUen_US
dc.subBiotechnology and Molecular Biology
dc.these.typeM.Sc
dc.titleDevelopment of micropropagation protocols in Cappris decidua (forsk) edgewen_US
dc.typeThesisen_US
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