Development of micropropagation protocols in Cappris decidua (forsk) edgew
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Date
2005
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Publisher
CCSHAU
Abstract
In vitro clonal propagation of Capparis decidua was achieved using
nodal part and shoot tip explants from mature trees grown in CCS
Haryana Agricultural University, Hisar Campus. Response for shoot
regeneration was observed within five days on all the media tested from
nodal and shoot tip explants. Shoot regeneration was observed only on
MS medium supplemented with 25.0 mg/L adenine sulphate and 5.0
mg/L BAP. Nodal explants gave best shoot regeneration (96%) on the
same MS medium supplemented with 25.0 mg/L adenine sulphate and
5.0 mg/L
BAP. On sub-culturign the explants on the same medium 10-11
shoots/explant were observed in 95% of explants cultured. Regenerated
shoots also subcultured on MS basal + culture supernatant of
Acetobacter (4 ml/L) and culture supernatant of Azotobacter 4 ml/L
medium formed multiple shoots in 82% of the cases with 14-16 shoots
per explant. These shoots were treated with liquid MS basal + 25.0
mg/L IBA for 6 days and then transferred to rooting media. Best rooting
was observed 37% on MS basal + 1.0 mg/L IBA and 1.0 mg/L IAA with
healthy and strong rooting. These shoots with roots were kept overnight
for hardening and then transferred to pots.