VARIABILITY PRESENT AMONG THE ISOLATES OF Pseudomonas spp. IN SAURASHTRA REGION OF GUJARAT 3748
dc.contributor.advisor | Dr. U. M. VYAS | |
dc.contributor.author | SAVITA PATIL | |
dc.contributor.other | 2010121085 | |
dc.date.accessioned | 2024-01-03T17:38:54Z | |
dc.date.available | 2024-01-03T17:38:54Z | |
dc.date.issued | 2023-08 | |
dc.description.abstract | Management of plant diseases is very important to attain a significant output in a crop production system. There is a great threat to the environment and crop ecosystem due to use of chemical pesticides even though they manage plant diseases effectively. Furthermore, many chemicals are suspected to harm non-targeted organisms and degradation of the environment especially soils. Biological control of plant pathogens by microorganisms and improvement of plant growth by enhancing nutrient uptake by roots is a potential non-chemical eco friendly tool. Members of the genus Pseudomonas have long been known for their potential to reduce the plant disease caused by fungal pathogens and they have gained considerable importance as potential antagonistic microorganisms. A survey was carried out from seven districts of Saurashtra region viz., Bhavanagar, Jamnagar, Rajkot, Porbandar, Junagadh, Gir Somnath and Amreli to collect soil samples from different fields of groundnut. Isolation and maintainance of isolates of Pseudomonas spp. was done by applying dilution plate technique. The colonies developed were yellowish with fluorescence under UV light. To study cultural variability the bacteria were grown on different media viz., Aleksandrow agar, King's B, Potato dextrose agar, Pikovaskay's agar, Pseudomonas agar, MacConkey Agar, and Nutrient agar medium. There was a difference in characteristics of colonies viz., bacterial growth, appearance, pigmentation, colony colour and fluorescence under UV light on seven different culture media. All the isolates of Pseudomonas spp. were screened for siderophore production by the CAS assay. Among thirty isolates tested, 27 isolates of Pseudomonas spp. were positive for siderophore production. Isolates Pf1, Pf5 and Pf12 were negative for siderophore production. Biochemical tests of the Pseudomonas isolates were done by using KB002 Hi Assorted Biochemical test kit (Himedia). The isolates Pf1, Pf10, Pf22 and Pf23 are identified as P. aeruginosa and isolates Pf3, Pf5, Pf9, Pf17, Pf18 and Pf30 are identified as P. putida and all the remaining isolates are characterized as P. fluorescens. Among the isolates of Pseudomonas spp. tested for their efficacy against the four soil borne fungal plant pathogens under in vitro condition, five isolates of Pf were effective in variable degrees against Aspergillus niger, Sclerotium rolfsii, Macrophomina phaseolina and Fusarium sp. Isolate Pf12 was most effective in inhibiting A. niger, whereas isolate Pf30 was less effective. Isolate Pf26 was most effective in inhibiting S. rolfsii, whereas, isolate Pf3 was less effective. Isolate Pf2 was most effective in inhibiting M. phaseolina, whereas, isolate Pf17 was less effective. Isolate Pf21 was most effective in inhibiting Fusarium sp. whereas, isolate Pf9 was less effective. Isolate Pf24 was found common among five highly antagonistic isolates in inhibiting mycelial growth of all four soil borne pathogens. Highly antagonistic Pseudomonas isolate i.e., Pf24 was tested for compatibility with fungicides, insecticides and weedicides at different concentrations. Among fungicides tested, the isolate was highly compatible with carbendazim followed by carbendazim 25% + mancozeb 50% WS. Whereas, hexaconazole was least compatible with isolate Pf24. Among insecticides tested, the isolate was highly compatible with fipronil followed by carbosulfan. Whereas, chlorpyriphos was least compatible with isolate Pf24. Among insecticides tested, the isolate was highly compatible with pendimethalin followed by quizalofop ethyl. Whereas, propaquizafop was least compatible with isolate Pf24. In pot culture studies the isolate Pf24 showed lowest disease incidence percentage of 45% compared to control in case of collar rot of groundnut. The isolates Pf2, Pf15 and Pf24 showed lowest disease incidence of 40% compared to control in case of root rot of chickpea. The isolates Pf4, Pf21 and Pf24 showed lowest disease incidence percentage of 50% compared to control in case of wilt of cumin | |
dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810205607 | |
dc.language.iso | English | |
dc.pages | 157 | |
dc.publisher | JAU JUNAGADH | |
dc.relation.ispartofseries | 3748 | |
dc.sub | Plant Pathology | |
dc.theme | VARIABILITY PRESENT AMONG THE ISOLATES OF Pseudomonas spp. IN SAURASHTRA REGION OF GUJARAT 3748 | |
dc.these.type | M.Sc | |
dc.title | VARIABILITY PRESENT AMONG THE ISOLATES OF Pseudomonas spp. IN SAURASHTRA REGION OF GUJARAT 3748 | |
dc.type | Thesis |