ISOLATION AND CHARACTERIZATION OF BACTERIOPHAGES AGAINST STAPHYLOCOCCUS SPECIES CAUSING MASTITIS IN BOVINES
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Date
2018-07
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KARNATAKA VETERINARY ANIMAL AND FISHERIES SCIENCE'SUNIVERSITY, BIDAR-585226
Abstract
Staphylococcus aureus is a common bacterium found on body of bovines but if it
bypasses the udder protection and enters the teat, it can cause mastitis which is a highly
economic important disease. In this study from 88 mastitis suspected milk samples, 45
Staphylococcus spps were isolated. Based on cultural, morphological and biochemical
characters, the isolates were found tentatively as Staphylococcus aureus, indicating
51.13% (46.66% in Cattle, 60.71% in Buffaloes) of Staphylococcus aureus mastitis in
bovine mastitis in Bidar district. Further the isolates were subjected for antibiogram study
and it was found that Staphylococcus aureus has developed Multi Drug Resistance to
many commonly used antimicrobials in the field. It is this antibiotic resistance problem,
which has made Staphylococcus aureus as one of the major hurdle for dairy farming all
over the world. To overcome this problem and to control and treat Staphylococcus aureus
mastitis, one such option is phage therapy. In this study 4 phages were isolated and
characterized from 32 sewage samples and tentatively named as SSPh1, SSPh2, SSPh3
and SSPh4. The phages SSPh1 and SSPh2 produced clear 1-2 mm plaques, whereas
phages SSPh3 and SSPh4 produced 2-3 mm clear plaques and all were able to produce good number of plaques at 108 to 1010 dilutions, indicating their high infective and strong
lytic character. On assessing the host range and killing potential of newly isolated phages,
it was found that all phages were narrow spectrum and Phage SSPh4 was found to be
monovalent in nature. None of the phages were able to lyse other species of bacteria
indicating their high specificity for Staphylococcus aureus. These isolated
Staphylococcus aureus phages can be potentially exploited for phage therapy as well as
for phage typing as an effective tool to control and treat Staphylococcus aureus mastitis
with further detailed study.
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