STUDIES ON CLOSTRIDIUM PERFRINGENS TYPE A NECROTIC ENTERITIS IN BROILERS
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Date
2016-07
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
The present study was undertaken to determine the diagnosis of Clostridium
perfringens type A in necrotic enteritis outbreaks in broilers. Necrotic enteritis in broilers
was diagnosed based on clinical signs, necropsy lesions, culture examination and
polymerase chain reaction. A total of 8 flocks with necrotic enteritis were selected and
per flock 6 samples from diseased birds and 6 samples from healthy birds were taken.
The intestinal contents or faecal sample was subjected for isolation and identification of
the bacteria using cooked meat medium and perfringens agar. Fifty Clostridium
perfringens isolates were obtained from 96 samples from diseased and healthy broiler
birds. Out of 48 isolates, 37 (75 %) isolates were from diseased broiler chicken and from
48 isolates, 13 (25%) isolates were from healthy broiler chicken. These isolates of
Clostridium perfringens were subjected to PCR and all the isolates produced the
amplification for alpha toxin gene and typed as Clostridium perfringens type A. The
morbidity and mortality rates varied between flocks, average morbidity rate was 25.9 per
cent and mortality rate was 6.9 per cent. Antibiotic sensitivity testing was carried out
using disc diffusion method on all isolates. Doxycycline showed maximum sensitivity
followed by ceftriaxone, lincomycin and virginiamycin. It is concluded that, isolation of
Clostridium perfringens was more in diseased birds than healthy birds and toxinotyping
of isolates by PCR technique is sensitive and rapid.