STUDIES ON HEMATO-BIOCHEMICAL PROFILE OF MICROSPORIDIAL AND CRYPTOSPORIDIAL INFECTIONS IN DOGS
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Date
2022
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
The Enterocytozoon bieneusi and Encephalitozoon intestinalis are most
common microsporidia infects the humans and have also been detected in dogs and
other animals causing gastrointestinal infection worldwide. Cryptosporidial infection
is a life-threatening in immunosuppressed patient such as HIV infected and organ
transplanted individuals. The studies have shown that dogs are intermittent shedders
of both microsporidia and Cryptosporidium in the environment and also with a known
fact that dogs are the most frequent close companion animals to humans posing a
threat of transmission of these pathogens. Hence the present study was undertaken
with an objective to investigate occurrence of microsporidia and Cryptosporidium and
their hemato-biochemical changes in dogs present around Shimogga district,
Karnataka. Upon examination of 124 faecal samples by using species specific PCR it
was found to be 4.83% prevalence rate of microsporidia more specifically
Enterocytozoon bieneusi. The prevalence rate varied between different places within
Shivamogga. From the PCR positive samples, pinkish spores can be appreciated
further by MT staining method. No samples were found positive for Cryptosporidium
by PCR method.
Hematological and biochemical parameters analyzed in microsporidia positive
samples revels there was a limited changes in lymphocyte count, ALT, creatinine and
total bilirubin concentration. The observations in the current study conclude that,
microsporidial infection was prevalent in the dogs of Karnataka. For the first time, the
zoonotic species, microsporidia Enterocytozoon bieneusi was recorded in dogs. As per
the available published literature, this study is the first of its kind on the prevalence of
microsporidia in the dogs in India.
Key words: microsporidia, Cryptosporidium, Enterocytozoon bieneusi, Modified
Trichrome (MT) stain, India