EVALUATION OF NANOPARTICLES FOR DETECTION OF ORGANOPHOSPHORUS PESTICIDE RESIDUES IN MILK
| dc.contributor.advisor | RAMAN SETH | |
| dc.contributor.author | WANGDARE SACHIN SUBHASH | |
| dc.date.accessioned | 2024-08-08T09:00:17Z | |
| dc.date.available | 2024-08-08T09:00:17Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | In twenty-first-century, organophosphate pesticides are being extensively used in the agricultural practices to increase the crop yield. Immeasurable use of these pesticides leads to variety of short-term and chronic adverse health implications. Most of the countries have banned these pesticides for agricultural purposes but they are still permitted in India. In many parts of India, milk was found to be contaminated with organophosphate pesticides particularly, chlorpyrifos and malathion. Source of contamination of these pesticides in milk is through feed and fodder. The present research work was aimed to develop gold nanoparticles (GNPs) based assay for detection chlorpyrifos and malathion pesticide in milk. Conventional assays already available for the detection of organophosphate pesticides such as GC-MS, LC-MS, and HPLC have been widely used. These methods however, require highly expensive instruments, tedious steps for sample preparation, time-consuming pretreatments and well trained technicians which limit their applications for on-site screening of these pesticides in milk. The development of present pesticide (chlorpyrifos and malathion) detection assay, required salt functionalization of GNPs was carried out using different salts and sodium sulphate was found to be excellent for functionalization of GNPs. The qualitative detection of chlorpyrifos and malathion was achieved up to the 15 and 30 ppb, respectively, using functionalized GNPs in pure acetonitrile system. Furthermore, limit of detection (LOD) of chlorpyrifos and malathion was achieved as 10.99 and 23.90 ppb, respectively in acetonitrile system. For milk system, qualitative detection of chlorpyrifos and malathion was achieved up to the 20 and 35 ppb, respectively, using functionalized GNPs. The limit of detection (LOD) of chlorpyrifos and malathion was achieved as 14.76 and 24.53 ppb, respectively, in milk system. The percent recovery of chlorpyrifos and malathion was 106.08 and 105.48, respectively, in milk system with developed assay. The HPLC-MS instrumental validation was also carried out in order to assess the analytical performance in detection of chlorpyrifos and malathion in milk using the GNPs based developed method. The LOD of HPLC-MS method for chlorpyrifos and malathion was found to be 7.58 and 3.21 ppb, respectively. The percent recovery of HPLC-MS method for chlorpyrifos and malathion from spiked raw milk samples were obtained as 102.13 and 101.84, respectively. It was found that both the methods were excellent for detection of chlorpyrifos and malathion pesticide in milk. The GNPs based developed present assay is very simple when compared with sophisticated instrumental assays. The developed GNPs based assay is very rapid, cost effective and reliable for qualitative as well as quantitative detection of chlorpyrifos and malathion pesticides in milk system. However, an inference of other prevalently occurring pesticides provides progressive future scope in the development of multi-pesticide detection assay for milk system using GNPs. | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810213048 | |
| dc.language.iso | English | |
| dc.pages | 118 p. | |
| dc.publisher | ICAR-NDRI, Karnal | |
| dc.sub | Food Technology | |
| dc.theme | FOOD SAFETY AND QUALITY ASSURANCE | |
| dc.these.type | Ph.D | |
| dc.title | EVALUATION OF NANOPARTICLES FOR DETECTION OF ORGANOPHOSPHORUS PESTICIDE RESIDUES IN MILK | |
| dc.type | Thesis |