CERTAIN STUDIES ON FELINE HAEMOTROPIC MYCOPLASMOSIS
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Date
2022
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
The present study “Certain studies on Feline Haemotropic Mycoplasmosis” was
designed to investigate epidemiological aspects, clinical and haemato-biochemical profile
associated with feline haemotropic mycoplasmosis and its molecular diagnosis. Among the
cats presented to Veterinary College Hospital, Bengaluru during the period from July 2020
to June 2021, 78 anaemic cats were suspected for Haemotropic Mycoplasmosis and were
included for detailed study. Peripheral blood smear stained with Giemsa stain were
examined for primary identification of organisms. Among 78 cats screened, 12 were
positive for haemoplasma by blood smear examination. Most frequent clinical signs
noticed in affected cats were pale mucous membranes, anorexia, lethargy, pyrexia, weight
loss and splenomegaly. Haematological studies revealed reduction in RBC, haemoglobin
and haematocrit values. Biochemical alterations were Hyperglobulinaemia and elevated
total protein level. Higher occurrence was observed in summer season and among adult
cats, male, non-descript cats with semi-outdoor access. The blood samples were subjected
to PCR for amplification of 16S rRNA gene of haemoplasma. The occurrence of
Mycoplasma haemofelis and Candidatus Mycoplasma haemominutum was five and
thirteen per cent, respectively. Phylogenetic analysis of Mycoplasma haemofelis derived
from the hemoplasma infected cats showed high sequence identity to M. haemocanis
sequences in Genbank and Candidatus Mycoplasma haemominutum showed 98 – 99 per
cent sequence identity to Candidatus Mycoplasma haemominutum isolates of domestic cats
from USA, Trinidad, Hungry, Italy, Yamaguchi, Brazil and Thaiwan. Statistically
significant reduction in plasma reduced glutathione concentration was seen and no
statistically significant difference in IgG quantity was observed between healthy and
infected groups of cats.
Key Words: Feline Haemotropic Mycoplasmosis, Blood smear, Polylerase Chain Reaction.
Annexures