CONSERVATION OF GEOGRAPHICAL INDICATION (GI) TAGGED JASMINES THROUGH TISSUE CULTURE
| dc.contributor.advisor | Nirmala, K. S. | |
| dc.contributor.author | RANGANATHA, G. J. | |
| dc.date.accessioned | 2020-10-07T06:09:02Z | |
| dc.date.available | 2020-10-07T06:09:02Z | |
| dc.date.issued | 2019-10-31 | |
| dc.description.abstract | Karnataka registered three jasmines cultivars in geographical indication registry during the year 2006. They are Mysore mallige (Jasminum azoricum L. syn. J. trifoliatum Moench), Udupi mallige (J. sambac (L.) Aiton) and Hadagali mallige (J. auriculatum Vahl), flowers of each of these species are unique in their fragrance. The present study aimed to conserve these species through slow growth storage, by direct and indirect organogenesis. In indirect organogenesis, the response of leaf explants for callus initiation was 100% in all the three species, early callus initiation being observed in MS medium supplemented with 2,4-D at 0.2 mg L-1 after 10, 11.6 and 9 days in Udupi mallige, Hadagali mallige and Mysore mallige respectively. Callus intensity was highest in the medium supplemented with 2,4-D at 0.4 mg L-1 : Udupi mallige (Very good), Hadagali mallige (Good), and Mysore mallige (Good). Callus was friable and white (Udupi mallige), brownish (Hadagali mallige) and creamish green (Mysore mallige). However, no organogenesis was observed when cultured on benzyl adenine (BA), kinetin (KN), naphthalin acetic acid (NAA) and in their combinations. The auxins (NAA (0.25 mg L-1 to 0.5 mg L-1 ) , 2,4-D (0.2 mg L-1 to 0.4 mg L-1 ) and cytokinin (Kinetin (2.0 mg L-1 to 6.0 mg L-1 ) , BA (2.0 mg L-1 to 6.0 mg L-1 ) treated cultures showed browning of callus after 60 days of culturing. For direct regeneration, shoot tips of all the three species were cultured on MS medium containing BA, KN, NAA and their combinations which resulted in only callus production. In none of the three species multiple shoots were noticed. Slow growth storage method adopted to conserve the callus, with 1.0 mg L-1 to7.5 mg L-1 ancymidol and 0.1 mg L-1 to 2.0 mg L-1 abscisic acid on half MS media indicated that the ancymidol treated cultures do not show any change even after 75 days, but abscisic acid treated cultures turned brown from white (Udupi mallige), brownish (Hadagali mallige) and creamish green (Mysore mallige) after 60 days. | en_US |
| dc.identifier.other | Th-12521 | |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810152703 | |
| dc.keywords | Jasmines ,Growth storage, Creamish green. | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 95 | en_US |
| dc.publisher | UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK BENGALURU | en_US |
| dc.sub | Horticulture | en_US |
| dc.theme | TAGGED JASMINES THROUGH TISSUE CULTURE | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | CONSERVATION OF GEOGRAPHICAL INDICATION (GI) TAGGED JASMINES THROUGH TISSUE CULTURE | en_US |
| dc.type | Thesis | en_US |