CONSERVATION OF GEOGRAPHICAL INDICATION (GI) TAGGED JASMINES THROUGH TISSUE CULTURE
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Date
2019-10-31
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UNIVERSITY OF AGRICULTURAL SCIENCES, GKVK BENGALURU
Abstract
Karnataka registered three jasmines cultivars in geographical indication registry
during the year 2006. They are Mysore mallige (Jasminum azoricum L. syn. J. trifoliatum
Moench), Udupi mallige (J. sambac (L.) Aiton) and Hadagali mallige (J. auriculatum
Vahl), flowers of each of these species are unique in their fragrance. The present study
aimed to conserve these species through slow growth storage, by direct and indirect
organogenesis. In indirect organogenesis, the response of leaf explants for callus initiation
was 100% in all the three species, early callus initiation being observed in MS medium
supplemented with 2,4-D at 0.2 mg L-1 after 10, 11.6 and 9 days in Udupi mallige, Hadagali
mallige and Mysore mallige respectively. Callus intensity was highest in the medium
supplemented with 2,4-D at 0.4 mg L-1 : Udupi mallige (Very good), Hadagali mallige
(Good), and Mysore mallige (Good). Callus was friable and white (Udupi mallige),
brownish (Hadagali mallige) and creamish green (Mysore mallige). However, no
organogenesis was observed when cultured on benzyl adenine (BA), kinetin (KN),
naphthalin acetic acid (NAA) and in their combinations. The auxins (NAA (0.25 mg L-1
to
0.5 mg L-1
) , 2,4-D (0.2 mg L-1 to 0.4 mg L-1 ) and cytokinin (Kinetin (2.0 mg L-1
to 6.0
mg L-1
) , BA (2.0 mg L-1
to 6.0 mg L-1
) treated cultures showed browning of callus after
60 days of culturing. For direct regeneration, shoot tips of all the three species were
cultured on MS medium containing BA, KN, NAA and their combinations which resulted
in only callus production. In none of the three species multiple shoots were noticed. Slow
growth storage method adopted to conserve the callus, with 1.0 mg L-1
to7.5 mg L-1
ancymidol and 0.1 mg L-1
to 2.0 mg L-1
abscisic acid on half MS media indicated that the
ancymidol treated cultures do not show any change even after 75 days, but abscisic acid
treated cultures turned brown from white (Udupi mallige), brownish (Hadagali mallige)
and creamish green (Mysore mallige) after 60 days.