STUDIES ON DIAGNOSIS OF HEPATIC INJURY IN DOGS
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Date
2022
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCESUNIVERSITY, BIDAR
Abstract
The present study titled “Studies on diagnosis of hepatic injury in dogs” carried
out at Veterinary College Hospital, Bengaluru with objective to study biomarkers and
ultrasonographic changes in hepatic injury in dogs and to determine the efficacy of
biomarkers in early diagnosis of hepatic injury. Based on ALT values, dogs were divided
into 3 groups of 6 animals each. Group I comprised of animals with clinical signs of liver
disorder with elevated ALT (>102 IU/L), Group II with clinical signs of liver disorder
with normal ALT levels (≤ 102 IU/L) and Group III was healthy control. Clinical signs
exhibited in Group I and II were anorexia, depression, lethargy, weight loss, vomiting,
diarrhea, polydipsia, polyuria, abdominal enlargement, behavioural changes and head
pressing. Haematological changes were reduced Hb, TEC, PCV, platelet count and
increased TLC in Group I, II compared to III. Biochemical changes were increased ALT,
AST, ALP, GGT, total and direct bilirubin and decreased total protein and albumin in
Group I, II compared to III. MicroRNA 122 was assessed by RT PCR in animals of
Group I, II, III. No significant difference in ct values of MicroRNA122 in all Groups.
Ultrasonography revealed marked changes in Group I, II compared to III. It was
concluded that combination of biomarkers should be used for early diagnosis and
progression of hepatic injury could be evaluated by any one or all of the biomarkers
along with ultrasonography. It was also found that MicroRNA 122 did not prove useful
for early diagnosis of hepatic injury in dogs.
Keywords: Hepatic injury, ALT, GGT, Hb, TEC, MicroRNA122, RT PCR