MOLECULAR DIVERSITY AND TAGGING OF RAPD MARKER TO MUNGBEAN YELLOW MOSAIC VIRUS (MYMV) RESISTANCE IN MUNGBEAN [Vigna radiata (L.) Wilczek]
| dc.contributor.advisor | SAVITHRAMMA, D. L. | |
| dc.contributor.author | PRAVEEN, B. HOLEYACHI. | |
| dc.date.accessioned | 2017-06-29T09:23:54Z | |
| dc.date.available | 2017-06-29T09:23:54Z | |
| dc.date.issued | 2009-09-10 | |
| dc.description.abstract | The present study was undertaken to identify RAPD marker associated with Mungbean Yellow Mosaic Virus (MYMV) resistance in mungbean using bulked segregant analysis in 93 F5 recombinant inbred lines (RILs) of the cross Chinamung x BL-849 which were sown in 3 replications.Observations for 11 morphological characters were recorded.. Thirty nine lines were found to be resistant, 18 lines were moderately resistant, 27 lines were moderately susceptible, 8 lines were susceptible and one line was highly susceptible to MYMV. Out of 20 primers used only ten primers showed polymorphism between parents Chinamung and BL 849. Only one primer UBC 499 out of 10 primers exhibited polymorphism between both parents, bulks and it amplified a single 700 bp band in resistant BL 849, resistant bulk and in individuals of F5 RILs constituting the resistant bulk which was absent in susceptible parent Chinamung and susceptible bulk indicating that primer UBC 499 was linked to MYMV resistance. This marker can be used in screening mungbean genotypes for resistance to MYMV disease. Genetic diversity studies in 28 mungbean cultivars using 15 RAPD primers, the dendrogram obtained by unweighted pair group using arithmetic mean (UPGMA) method resolved the cultivars into five major clusters. Squared euclidean distance ranged from 3 to 59 indicating presence of diversity among 28 genotypes. In an separate experiment involving F4 and F5 generation of three crosses in mungbean, all the characters studied showed nonsignificant correlation between F4-F5 generation except plant height and number of pods per cluster which showed a positive significant association in the cross PS 16 x KM-1883 and selection for these characters is worth attempting. | en_US |
| dc.identifier.other | Th-9504 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810023709 | |
| dc.language.iso | en | en_US |
| dc.pages | 177 | en_US |
| dc.publisher | University of Agricultural Sciences GKVK, Bangalore | en_US |
| dc.sub | Genetics and Plant Breeding | en_US |
| dc.subject | null | en_US |
| dc.theme | DIVERSITY AND TAGGING OF RAPD MARKER TO MUNGBEAN YELLOW MOSAIC VIRUS | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | MOLECULAR DIVERSITY AND TAGGING OF RAPD MARKER TO MUNGBEAN YELLOW MOSAIC VIRUS (MYMV) RESISTANCE IN MUNGBEAN [Vigna radiata (L.) Wilczek] | en_US |
| dc.type | Thesis | en_US |