MOLECULAR DIVERSITY AND TAGGING OF RAPD MARKER TO MUNGBEAN YELLOW MOSAIC VIRUS (MYMV) RESISTANCE IN MUNGBEAN [Vigna radiata (L.) Wilczek]
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Date
2009-09-10
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University of Agricultural Sciences GKVK, Bangalore
Abstract
The present study was undertaken to identify RAPD marker associated
with Mungbean Yellow Mosaic Virus (MYMV) resistance in mungbean using
bulked segregant analysis in 93 F5 recombinant inbred lines (RILs) of the cross
Chinamung x BL-849 which were sown in 3 replications.Observations for 11
morphological characters were recorded.. Thirty nine lines were found to be resistant,
18 lines were moderately resistant, 27 lines were moderately susceptible, 8 lines were
susceptible and one line was highly susceptible to MYMV. Out of 20 primers used
only ten primers showed polymorphism between parents Chinamung and BL 849.
Only one primer UBC 499 out of 10 primers exhibited polymorphism between both
parents, bulks and it amplified a single 700 bp band in resistant BL 849, resistant bulk
and in individuals of F5 RILs constituting the resistant bulk which was absent in
susceptible parent Chinamung and susceptible bulk indicating that primer UBC 499
was linked to MYMV resistance. This marker can be used in screening mungbean
genotypes for resistance to MYMV disease. Genetic diversity studies in 28 mungbean
cultivars using 15 RAPD primers, the dendrogram obtained by unweighted pair group
using arithmetic mean (UPGMA) method resolved the cultivars into five major
clusters. Squared euclidean distance ranged from 3 to 59 indicating presence of
diversity among 28 genotypes. In an separate experiment involving F4 and F5
generation of three crosses in mungbean, all the characters studied showed nonsignificant
correlation between F4-F5 generation except plant height and number of
pods per cluster which showed a positive significant association in the cross PS 16 x
KM-1883 and selection for these characters is worth attempting.
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