MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF ENDOPHYTIC BACTERIA ASSOCIATED WITH AEROBIC RICE AND THEIR ROLE IN PLANT GROWTH
| dc.contributor.advisor | Shivaprakash, M. K. | |
| dc.contributor.author | AMANDA, SHYLLA | |
| dc.date.accessioned | 2018-03-01T04:11:53Z | |
| dc.date.available | 2018-03-01T04:11:53Z | |
| dc.date.issued | 2016-10-07 | |
| dc.description.abstract | Bacterial endophytes occur inside a plant and act as plant growth promoting bacteria. The present study was carried out to isolate and characterize endophytic bacteria residing inside the aerobic rice varieties and evaluate their role as plant growth promoters. Twenty-four endophytic bacteria were isolated from the leaves, stems, and roots of four aerobic rice genotype. The diversity study was carried out by culture dependent and culture independent techniques. Through culture based method four genera were identified and were further clarified by culture independent method. The genera identified are Pantoea, Pseudomonas, Serratia, Bacillus, Achromobacter, Brevundimonas, and two other genera which were yet to be characterized. Metagenomic work was carried to get clear picture of diversity of bacterial endophytes. Through 16S rRNA sequence using specific primers, it was confirmed that there are ten genera associated with the aerobic rice variety. Most of the isolated endophytes showed plant growth-promoting (PGP) ability like nitrogen fixing ability, phosphate solubilizing activity and production of gibberellic acid (GA), indole acetic acid (IAA), cytokinin, 1- aminocyclopropane-1-carboxylate (ACC) and siderophores. Four isolates showed antagonistic effect against Rhizoctonia solani and the best isolates was identified as Pseudomonas sp. Three isolates Pseudomonas sp, Serratia sp and Pantoea sp were selected for greenhouse study. These bacterial endophytes either individually or in combination with reduced amount of fertilizers improved plant growth and yield of the aerobic rice. These results strongly suggest that the endophytic bacteria characterized in this study could be successfully used to promote plant growth and inducing fungal resistance in plant. | en_US |
| dc.identifier.other | Th-11529 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810041449 | |
| dc.language.iso | en | en_US |
| dc.pages | 178 | en_US |
| dc.publisher | UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU | en_US |
| dc.sub | Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | MOLECULAR AND BIOCHEMICAL CHARACTERIZATION | en_US |
| dc.these.type | Ph.D | en_US |
| dc.title | MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF ENDOPHYTIC BACTERIA ASSOCIATED WITH AEROBIC RICE AND THEIR ROLE IN PLANT GROWTH | en_US |
| dc.type | Thesis | en_US |