MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF ENDOPHYTIC BACTERIA ASSOCIATED WITH AEROBIC RICE AND THEIR ROLE IN PLANT GROWTH
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Date
2016-10-07
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Bacterial endophytes occur inside a plant and act as plant growth promoting
bacteria. The present study was carried out to isolate and characterize endophytic bacteria
residing inside the aerobic rice varieties and evaluate their role as plant growth
promoters. Twenty-four endophytic bacteria were isolated from the leaves, stems, and
roots of four aerobic rice genotype. The diversity study was carried out by culture
dependent and culture independent techniques. Through culture based method four
genera were identified and were further clarified by culture independent method. The
genera identified are Pantoea, Pseudomonas, Serratia, Bacillus, Achromobacter,
Brevundimonas, and two other genera which were yet to be characterized. Metagenomic
work was carried to get clear picture of diversity of bacterial endophytes. Through 16S
rRNA sequence using specific primers, it was confirmed that there are ten genera
associated with the aerobic rice variety. Most of the isolated endophytes showed plant
growth-promoting (PGP) ability like nitrogen fixing ability, phosphate solubilizing
activity and production of gibberellic acid (GA), indole acetic acid (IAA), cytokinin, 1-
aminocyclopropane-1-carboxylate (ACC) and siderophores. Four isolates showed
antagonistic effect against Rhizoctonia solani and the best isolates was identified as
Pseudomonas sp. Three isolates Pseudomonas sp, Serratia sp and Pantoea sp were
selected for greenhouse study. These bacterial endophytes either individually or in
combination with reduced amount of fertilizers improved plant growth and yield of the
aerobic rice. These results strongly suggest that the endophytic bacteria characterized in
this study could be successfully used to promote plant growth and inducing fungal
resistance in plant.
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