TOXICITY STUDY OF FUNGAL CONTAMINATED BREWER’S SPENT GRAIN IN RATS
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Date
2023
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
The present study was undertaken to evaluate the toxicity of fungal
contaminated brewer’s spent grain in rats. Repeated dose 28-day oral toxicity study of
mycotoxins present in mixed fungal culture filtrates of Rhizopus oryzae 25%,
Aspergillus flavus 50% and Aspergillus nomius 25%, was conducted by gavaging it to
rats. 40 Wister albino rats were divided in to four groups, 10 rats in each group of
either sex. Keeping one group as control, other three groups received the mixed
fungal culture filtrates at low dose (0.5 ml/100 g), medium dose (1 ml/100 g) and high
dose (2 ml/100 g) rates depending on their body weights respectively. Body weight
parameter and blood samples were taken on day 0, 14 and 28 days of the study.
Biochemical parameters like ALT, AST, BUN, creatinine and haematological
parameters like TLC, TEC, Hb, PCV and Blood clotting time were analysed.
Significant (P<0.05) decrease in body weight, significant (P<0.05) increase in ALT,
AST, BUN and Creatinine serum concentration, a significant (P<0.05) fall in TLC,
TEC, Hb and PCV with significant (P<0.05) increase in blood clotting time in
treatment groups compared to control group rats were observed. The gross and
histopathological changes revealed, hepatotoxicity, nephrotoxicity, immunotoxicity,
and cardiotoxicity in all the treatment groups. Thus, it was concluded from the
observations in the present study that, the fungal culture filtrate had shown toxicity in
rats, attributed to the presence of toxic principle in mixed fungal culture filtrates.
Key words: Brewer’s spent grain, Toxicity, Rat, Rhizopus oryzae, Aspergillus flavus,
Aspergillus nomius