“EFFECT OF GIBBERELLIC ACID, POTASSIUM NITRATE AND SILICIC ACID ON BIOCHEMICAL CHANGES IN GROUNDNUT (Arachis hypogaea L.) SEEDLING IRRIGATED WITH SALINE WATER” 2885
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Date
2019-07
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JAU, JUNAGADH
Abstract
Green house experiment was conducted to investigate the effect of exogenous
application of gibberellic acid, potassium nitrate and silicic acid under salt stress on
physiological, biochemical parameters and enzymatic activities of groundnut. The
experimental design was factorial completely randomized design with different eight (T1
to T8) treatment combinations, two level of irrigation to induce salinity stress i.e. tap
water (S1) and 4 EC (S2). The plants were sprayed with GA3, KNO3 and silicic acid after
20 and 40 DAS (Days after sowing) and samples withdrawn after 30 and 50 DAS for the
analysis. The observations were recorded for the parameters viz., relative water content,
leaf pH, membrane stability index, total phenol, true protein, free amino acid, total
soluble sugar, reducing sugar, chlorophyll content, carotenoids, proline, glycine betaine
as well as different enzymes viz., polyphenol oxidase, peroxidase and catalase.
The present investigation was under taken with an objective to find out the effect
of exogenous application of gibberellic acid, potassium nitrate and silicic acid as growth
regulators on physiological, biochemical and enzymatic activities. Results revealed that
the foliar spray of treatment T8 [GA3 @ 100 ppm + KNO3 @ 500 ppm + silicic acid @ 50
ppm] was more effective in all parameters.
In present study, it was observed that increasing the salt concentration in
treatments resulted to increase osmolytes or solutes viz., total soluble sugar, reducing
sugar, glycine betain, total phenols and proline content in groundnut under salt stress.Abstract
With progressive days of germination, osmolytes or solute content in the seedling
decrease in all salt stress treatments. A difference in sensitivity to salinity in groundnut
was also reflected on physiological parameters as well as for the enzymatic activity. Salt
stress that decreased physiological parameters like; relative water content and membrane
stability index but leaf pH increased under salt stress.
The leaf sample that treated with gibberellic acid, potassium nitrate and silicic
acid at different concentrations that increased physiological parameters like relative water
content, membrane stability index, except leaf pH. GA3, KNO3, silicic acid possibly
regulate various plant metabolic process and modulate the production of varied osmolytes
like total phenol, free amino acid, total soluble sugar, reducing sugar, chlorophyll content
(a, b and total), proline and glycine betaine, enzyme activity like peroxidase increased
under salt stress, but application of GA3, KNO3 and silicic acid decrease catalase and
polyphenol oxidase activity.
This investigation has proved GA3, KNO3 and silicic acid as potential
biomolecules in reducing the adverse effect of salt stress in plant. GA3, KNO3 and silicic
acid has been showed to be beneficial for plant growth and development.