NUCLEIC ACID BASED DETECTION OF MYCOBACTERIUM AVIUM SUBSP PARATUBERCULNSIS (MAP), LEPTOSPIRA AND GENETIC SUSCEPTIBILITY TO MAP

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dc.contributor.advisorRaj, G. Dhinakar
dc.contributor.advisorSrinivasan, S.R.
dc.contributor.advisorKumanan, K.
dc.contributor.advisorRavikumar, G.
dc.contributor.authorAnitha, S.
dc.contributor.authorTANUVAS
dc.date.accessioned2018-02-09T06:46:23Z
dc.date.available2018-02-09T06:46:23Z
dc.date.issued2015
dc.descriptionTNV_TH_2015_DPV(M)09001en_US
dc.description.abstractThe present study was undertaken to evolve nucleic acid detection methodology that Would prevent aerosol contamination of the previous amplified product and non-specific amplification. The approach used in this study for achieving the above objectives were to incorporate dUTP in the place of dTTP and perform subsequent polymerase chain reaction (PCR) or loop mediated isothermal amplification (LAMP) reaction in the presence of enzyme Uracil DNA glycosylase and to use the lateral flow dipstick assay on the LAMP products. These two approaches are applied in the diagnosis of Leptospira and Mycobacterium avíum subsp paratuberculosis (MAP).en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810040750
dc.keywordsNucleic Acid Based Detection - Mycobacterium Avium Subsp - Paratuberculnsis (Map), Leptospira - Genetic Susceptibility - Mapen_US
dc.language.isoenen_US
dc.pages1-4en_US
dc.publisherTANUVAS, Chennaien_US
dc.subAnimal Biotechnologyen_US
dc.subjectVeterinary Scienceen_US
dc.subjectAnimal Biotechnologyen_US
dc.themeAnimal Biotechnologyen_US
dc.these.typePh.Den_US
dc.titleNUCLEIC ACID BASED DETECTION OF MYCOBACTERIUM AVIUM SUBSP PARATUBERCULNSIS (MAP), LEPTOSPIRA AND GENETIC SUSCEPTIBILITY TO MAPen_US
dc.typeThesisen_US
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