NUCLEIC ACID BASED DETECTION OF MYCOBACTERIUM AVIUM SUBSP PARATUBERCULNSIS (MAP), LEPTOSPIRA AND GENETIC SUSCEPTIBILITY TO MAP
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Date
2015
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
TANUVAS, Chennai
Abstract
The present study was undertaken to evolve nucleic acid detection
methodology that Would prevent aerosol contamination of the previous amplified
product and non-specific amplification. The approach used in this study for
achieving the above objectives were to incorporate dUTP in the place of dTTP and
perform subsequent polymerase chain reaction (PCR) or loop mediated isothermal
amplification (LAMP) reaction in the presence of enzyme Uracil DNA glycosylase
and to use the lateral flow dipstick assay on the LAMP products. These two
approaches are applied in the diagnosis of Leptospira and Mycobacterium avíum
subsp paratuberculosis (MAP).
Description
TNV_TH_2015_DPV(M)09001
Keywords
Veterinary Science, Animal Biotechnology