STUDIES ON MAJOR BACTERIAL-VIRAL AGENTS ASSOCIATED WITH DIARRHOEA IN CALVES

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dc.contributor.advisorARUN S. J.)
dc.contributor.authorVEENAKUMARI A. N.
dc.date.accessioned2023-12-22T11:20:47Z
dc.date.available2023-12-22T11:20:47Z
dc.date.issued2022
dc.description.abstractCalf diarrhoea is a commonly reported disease of calves caused by multietiological agents leading to high morbidity and mortality. The present study was carried out to isolate and characterize predominant bacteria such as E. coli and Salmonella, and to detect bovine rotavirus and bovine coronavirus in the faecal samples of diarrhoeic and non-diarrhoeic calves. For this a total of 162 faecal samples were collected from diarrhoeic and non-diarrhoeic calves under six months of age. In total, 155 and 8 faecal samples were positive for E. coli and Salmonella respectively. Duplex PCR was performed to confirm E. coli by targeting the uidA and uspA genes and uniplex PCR was performed to confirm Salmonella by targeting the invA gene. The virulence genes of E. coli, namely, fimH, stx1 and stx2 were detected in 94.19% (146/155), 25.16% (39/155) and 32.25% (50/155), respectively. All the Salmonella isolates in our study were found to be S. Typhimurium 100% (8/8) and none were S. Enteritidis. The stn gene was detected in 87.5% (7/8) of Salmonella isolates. All 162 faecal samples were also screened for the presence of bovine rotavirus and bovine coronavirus by RT-PCR by targeting VP6 and N genes, respectively. Bovine rotavirus was detected in 47.53% (77 out of 162) samples, whereas none of the faecal samples were positive for bovine coronavirus. The antibiogram study by disc diffusion method revealed that the bacterial species were 100% resistant to metronidazole and polymyxin-B, but susceptible to ciprofloxacin and co-trimoxazole. The study gave an insight into the major bacterial and viral etiological profile, antibiogram and virulence potential of bacterial agents associated with calf diarrhoea. Key words: Calf diarrhoea, E. coli, Salmonella serovars, bovine rotavirus, bovine coronavirus, antibiogram
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810204204
dc.language.isoEnglish
dc.pages285
dc.publisherKARNATAKA VETERINARY, ANIMAL ANDFISHERIES SCIENCES UNIVERSITY, BIDAR
dc.subVeterinary Microbiology
dc.themeVeterinary Microbiology
dc.these.typeM.V.Sc.
dc.titleSTUDIES ON MAJOR BACTERIAL-VIRAL AGENTS ASSOCIATED WITH DIARRHOEA IN CALVES
dc.typeThesis
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