In vitro PROPAGATION AND CONSERVATION OF Swertia chirayita

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dc.contributor.advisorKANWAR, KAMLESH
dc.contributor.authorSHAILJA
dc.date.accessioned2017-05-15T11:09:18Z
dc.date.available2017-05-15T11:09:18Z
dc.date.issued2013
dc.description.abstractAbstract A protocol for in vitro propagation and conservation was developed for Swertia chirayita, an endangered medicinal plant. The sterilized explants (leaves) cultured on MS medium supplemented with 0.1 mg/l NAA and 3.0 mg/l BA gave best results for in vitro callus induction. Shoot regeneration was obtained from the callus on the same medium. The in vitroshoots cultured on MS medium supplemented with 2.5mg/l BA and 0.1 mg/l Kinetin gave best results for in vitro shoot multiplication. The MS medium supplemented with 0.1 mg/l NAA and 3.0 mg/l BA medium was found to be thebest for direct shoot regeneration from in vitroleaves. 80.30% root induction from in vitro grown shoots was obtained on half strength MS medium supplemented with 400 mg/l activated charcoal and 0.1 mg/l NAA. In vitro conservation was carried out by using two different approaches namely slow growth by changing media composition (sucrose and abscisic acid), at low temperature and cryopreservation following vitrification.With increase in concentration of sucrose and ABA decrease in growth of in vitroshoots was observed. No shoot multiplication with average leaf size of 0.35 cm and shoot length 0.67 cm was observed on half MS containing 90 g/l sucrose. Similarily, in case of media containing half strength MS salts and 3.0 mg/l ABA showed no shoot multiplication,0.83 cm average leaf size and 0.83 cm shoot length. At low temperature the in vitro shoots incubated at 4 o C, showed 100% retrieval, with 1.00 cm average number of shoots, 0.86 cm shoot length and 0.34 cm leaf size. In vitro shoots incubated at 10 o C, showed 100% retrieval, with 1.00 cm average number of shoots, 0.76 cm shoot length and 0.23 cm leaf size. During studies the vitrified shoot gave retrieval of 42.33% when precooled at 4 o C while only 22.37% vitrified shoots were retrieved from those precooled at 10 o C.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810011197
dc.keywordsSwertia chirayita, in vitro propagation,conservationen_US
dc.language.isoenen_US
dc.publisherUHF,NAUNIen_US
dc.subBiotechnologyen_US
dc.subjectnullen_US
dc.themeSwertia chirayita, in vitro propagation,conservationen_US
dc.these.typeM.Scen_US
dc.titleIn vitro PROPAGATION AND CONSERVATION OF Swertia chirayitaen_US
dc.typeThesisen_US
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