In vitro PROPAGATION AND CONSERVATION OF Swertia chirayita
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Date
2013
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Publisher
UHF,NAUNI
Abstract
Abstract
A protocol for in vitro propagation and conservation was developed for Swertia
chirayita, an endangered medicinal plant. The sterilized explants (leaves) cultured on MS
medium supplemented with 0.1 mg/l NAA and 3.0 mg/l BA gave best results for in vitro
callus induction. Shoot regeneration was obtained from the callus on the same medium. The
in vitroshoots cultured on MS medium supplemented with 2.5mg/l BA and 0.1 mg/l Kinetin
gave best results for in vitro shoot multiplication. The MS medium supplemented with 0.1
mg/l NAA and 3.0 mg/l BA medium was found to be thebest for direct shoot regeneration
from in vitroleaves. 80.30% root induction from in vitro grown shoots was obtained on half
strength MS medium supplemented with 400 mg/l activated charcoal and 0.1 mg/l NAA.
In vitro conservation was carried out by using two different approaches namely slow growth
by changing media composition (sucrose and abscisic acid), at low temperature and
cryopreservation following vitrification.With increase in concentration of sucrose and ABA
decrease in growth of in vitroshoots was observed. No shoot multiplication with average leaf
size of 0.35 cm and shoot length 0.67 cm was observed on half MS containing 90 g/l sucrose.
Similarily, in case of media containing half strength MS salts and 3.0 mg/l ABA showed no
shoot multiplication,0.83 cm average leaf size and 0.83 cm shoot length. At low temperature
the in vitro shoots incubated at 4
o
C, showed 100% retrieval, with 1.00 cm average number of
shoots, 0.86 cm shoot length and 0.34 cm leaf size. In vitro shoots incubated at 10
o
C, showed
100% retrieval, with 1.00 cm average number of shoots, 0.76 cm shoot length and 0.23 cm
leaf size. During studies the vitrified shoot gave retrieval of 42.33% when precooled at 4
o
C
while only 22.37% vitrified shoots were retrieved from those precooled at 10
o
C.
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