Agrobacterium mediated transformation in Phlogacanthus thyrsiflorus Nees for quality parameters
| dc.contributor.advisor | Banerjee, Madhuparna | |
| dc.contributor.author | KAMAL, SWATI | |
| dc.date.accessioned | 2017-07-27T07:04:58Z | |
| dc.date.available | 2017-07-27T07:04:58Z | |
| dc.date.issued | 2011 | |
| dc.description | Agrobacterium mediated transformation in Phlogacanthus thyrsiflorus Nees for quality parameters | en_US |
| dc.description.abstract | on P. thyrsiflorus which has not been reported earlier. The total carbohydrate content in hairy roots were also quantified and compared from leaves from mother plant and bacteria infected plant. Aseptic explants for transformation were obtained by inoculating shoot tip of P.thyrsiflorus on MS media supplemented with BAP 5.0 mg/l and AdSO4 50.0 mg/l. Leaves , shoot tips and nodes of in vitro grown plants were used as explants while ATCC 15834 strain of A. rhizogenes Conn was used for transformation. Full loop inocula of A.rhizogenes were grown in YEB (Yeast Extract Beef Extract) medium supplemented with 50 mg/l rifampicin. Optimum growth was observed after 48 h in dark at 28°C. Acclimatization of bacteria culture in MS medium for 4 h was found effective. The explants were treated for different time periods after which those were inoculated in solid MS basal medium and kept at 25 ± 2 °C and 16 h light/ 8 h dark. Successful hairy root induction was observed after 7 days on all 3 explants. Amongst different treatment durations, 225 min was found to be the best for all the three explants. A clear variation in response was observed within different types of explants. Out of three explants, shoot tips were found to be the best with maximum mean percentage of root induction (93.33%) as well as maximum mean number of roots (4.80) per explant. Total carbohydrate content was quantified with anthrone method which revealed that the carbohydrate content was highest (2.9 mg percent) in hairy roots followed by the leaves from bacteria infected plant (2.0 mg percent). However, leaves of mother plant showed comparatively minimum (1.6 mg percent) carbohydrate content. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810026272 | |
| dc.keywords | Agrobacterium mediated transformation in Phlogacanthus thyrsiflorus Nees for quality parameters | en_US |
| dc.language.iso | en_US | en_US |
| dc.pages | 53 | en_US |
| dc.publisher | Birsa Agricultural University, Kanke, Ranchi, Jharkhand | en_US |
| dc.sub | Biotechnology | en_US |
| dc.subject | null | en_US |
| dc.theme | Agrobacterium mediated transformation in Phlogacanthus thyrsiflorus Nees for quality parameters | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | Agrobacterium mediated transformation in Phlogacanthus thyrsiflorus Nees for quality parameters | en_US |
| dc.type | Thesis | en_US |