DEVELOPMENT OF FORMALIN FREE PRESERVATION OF BIOLOGICAL SPECIMENS
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Date
2022
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KARNATAKA VETERINARY, ANIMAL AND FISHERIES SCIENCES UNIVERSITY, BIDAR
Abstract
Glycerine dry mount technique can be an innovative method of preserving biological specimens. In the present study, the specimens like liver, spleen, lung along with trachea, heart, a pair of kidneys, brain and poultry visceral organs were collected from slaughter house and dog limbs were obtained from Department of Veterinary Anatomy, Bengaluru. Dehydration was carried out in three changes of pure acetone for three weeks depending on size of the specimen and impregnation was carried out using glycerine for 3-4 weeks at four different conditions and divided into four groups. The group I organs were dehydrated in normal room temperature and impregnated under forced vacuum. Maximum shrinkage was noticed in brain and minimum in liver. The group II organs were dehydrated in normal room temperature and impregnated under normal atmospheric pressure. Maximum shrinkage was noticed in kidneys and minimum in liver. The group III organs were dehydrated in refrigeration temperature and impregnated under forced vacuum. Maximum shrinkage was seen in poultry viscera (in situ) followed by brain and least by the liver. The group IV organs were dehydrated in refrigeration temperature and impregnated under normal atmospheric pressure. Maximum shrinkage was shown by spleen followed by brain and least by the poultry viscera. The consistency of all organs were soft but there was no change in any morphological details. From economic point of view, the cost of production of each group of specimens was approximately Rs.4000 per group without considering the labour and one-time investment of equipments. The glycerine dry mounted tissue sections were also microscopically evaluated and found compactness and distortion of the cellular structures and poor staining charactertics.
Key words: Glycerine dry mount, vacuum, temperature, shrinkage.