IDENTIFICATION AND CHARACTERIZATION OF MUSHROOM GROWTH PROMOTING BACTERIA FROM CASING SOIL AND THEIR EFFECT ON YIELD AND QUALITY OF BUTTON MUSHROOM

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dc.contributor.advisorDharmesh Gupta
dc.contributor.authorJYOTI NUGHAL
dc.date.accessioned2024-06-26T14:18:25Z
dc.date.available2024-06-26T14:18:25Z
dc.date.issued2024-05-25
dc.description.abstractGlobally, Agaricus bisporus (Lange) Imbach is cultivated as a food source, undergoing a crucial transition from vegetative to reproductive stages facilitated by a specific casing layer. In the present investigation 90 bacterial isolates from casing soil were collected and screened to see the effect of mushroom growth promoting bacteria (MGPB) on quality and yield of Agaricus bisporus (Lange) Imbach. The bacterial isolates were isolated on nutrient agar medium. Out of 90 isolates, 52 were selected for exhibiting all the traits associated with Mushroom Growth Promotion (MGP). SNG9 exhibited (226.39%) maximum qualitative P-solubilization and PRG10 showed maximum (173.51 μg/ml) quantitative P-solubilization. SNG10 demonstrated maximum siderophore production efficiency (158.15%) and per cent siderophore unit (201.57%). SNG10 also produced the highest IAA (41.59 μg/ml), while SNG9 had maximum gibberellic acid (55.31 μg/ml) and 85.56 per cent isolates demonstrated nitrogen-fixing ability. Chitinase activity was observed in 53.85 per cent of isolates, with PRG10 (4.22) having the highest enzyme index. Fifty per cent showed cellulase activity and KLH8 (5.55) exhibited the highest enzyme index. Protease activity was observed in 55.77 per cent with PRG10 having the highest enzyme index (4.43). Twelve isolates produced HCN and six showed antagonistic effects (>25%) against Trichoderma harzianum. Five highly effective bacterial antagonists exhibited >25% antagonistic effects against Mycogone perniciosa. Through morphological, biochemical and molecular characterization, the bacterial isolates were identified as Stenotrophomonas rhizophila (PRG10), Sphingobacterium nematocida (SNG9), Pseudomonas putida (SNG10) and Bacillus proteolyticus (KLH8). In field experiments, these isolates, either individually or in combination (PRG10, SNG9, SNG10 and KLH8) at concentrations of 2.5, 5, 7.5 and 10 ml were tested for white button mushroom growth. Treatments like PRG10+SNG9 at 5ml, SNG9+SNG10 at 5ml, SNG10+KLH8 at 7.5ml and KLH8 at 7.5ml exhibited a significant increase in yield (31.17%, 24.58%, 21.48% and 18.79%) and various growth parameters compared to the uninoculated control. Moreover, these treatments improved the physio-chemical properties of the casing soil, contributing to enhanced nutritional status in mushrooms. Therefore, these bacterial isolates possess significant potential for enhancing the yield and quality of button mushrooms.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810210950
dc.keywordsBUTTON MUSHROOM
dc.keywordsIDENTIFICATION AND CHARACTERIZATION OF MUSHROOM GROWTH PROMOTING BACTERIA FROM CASING SOIL
dc.language.isoEnglish
dc.pages172+viii
dc.publisherUHF Nauni
dc.subPlant Pathology
dc.theme“identification and characterization of mushroom growth promoting bacteria from casing soil and their effect on yield and quality of button mushroom
dc.these.typePh.D
dc.titleIDENTIFICATION AND CHARACTERIZATION OF MUSHROOM GROWTH PROMOTING BACTERIA FROM CASING SOIL AND THEIR EFFECT ON YIELD AND QUALITY OF BUTTON MUSHROOM
dc.typeThesis
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