DETECTION OF PROTEUS MIRABILIS IN FOODS OF ANIMAL ORIGIN, ANIMAL AND HUMAN CLINICAL SAMPLES AND WATER WITH SPECIAL EMPHASIS ON β-LACTAMASES
| dc.contributor.advisor | VENKATESWARA RAO, L(MAJOR) | |
| dc.contributor.advisor | SRINIVASA RAO, T | |
| dc.contributor.advisor | SUBRAMANYAM, K.V. | |
| dc.contributor.author | PRASASTHA RAM, V | |
| dc.date.accessioned | 2019-07-09T08:33:49Z | |
| dc.date.available | 2019-07-09T08:33:49Z | |
| dc.date.issued | 2018-12 | |
| dc.description | THESES | en_US |
| dc.description.abstract | P. mirabilis is an emerging foodborne pathogen having worldwide public health concern. The present study was undertaken to characterize P. mirabilis species of animal and human origin based on cultural isolation, PCR detection, antibiogram, virulence profiles and genetic diversity. A total of 507 samples comprising foods of animal origin (215), faecal swab samples (188), human urine samples (65), human diarrhoeic stool samples (12) as well as water samples (27) were examined. Overall prevalence of P. mirabilis was found to be 34.51% (175/507) by species-specific PCR. Among foods of animal origin, the highest rate of P. mirabilis isolates were recovered from chicken samples (38.7%), followed by pork (37.5%) and mutton samples (28.9%). Among faecal swabs of livestock, the highest rate of P. mirabilis isolates were recovered from poultry (49%), followed by pigs (37.8%). Human urine samples showed a prevalence rate of 10.7%. Water samples showed 7.4% prevalence. All the human diarrhoeic stool samples were negative for P. mirabilis. All the P. mirabilis isolates carried a combination of putative virulence genes. The genes ureC, ureA, flaA, hpmA and zapA were detected in 80.5%, 72.5%, 28.5%, 60.5% and 50.28% of P. mirabilis isolates, respectively. Antibiogram of P. mirabilis isolates revealed sensitivity towards gentamicin (76.57%), followed by ampicillin (64.57%), kanamycin (61.14%), amikacin (60.57%) and streptomycin (43.42%). Higher resistance was observed for erythromycin (71.42%), nalidixic acid (62.85%), ciprofloxacin (62.85%), tetracycline (60%), polymyxin-B (60%), cefoxitin (49.14%) and amikacin (36%). Notable percentages of isolates were intermediately resistant against streptomycin (33.14%), erythromycin (20.57%) and cefoxitin (18.28%). β-lactamase genes were detected in a total of 23 isolates (13.14%). Prevalence rates of β-lactamase genes among different samples was 23.6%, 11.1%, 10.8% and 42.8% from chicken, pork, poultry cloacal swabs and human urine samples, respectively with blaTEM being the predominant gene detected (69.56%) followed by blaOXA (26.08%), blaAmpC gene FOX (13.04%), blaCTX-M group I (4.34%), blaSHV (4.34%) and blaAmpC gene CIT (4.34%) among all the tested P. mirabilis isolates. Of the twenty-three P. mirabilis isolates analyzed, twenty-three ERIC-PCR patterns and twenty-two REP-PCR patterns were obtained. A pair of P. mirabilis isolates (13 and 14) that had identical REP-PCR pattern (R13) were distinguished by ERIC-PCR into two different genotypes (E13 and E14). The two P. mirabilis isolates sharing identical REP-PCR pattern (R13) but differential ERIC-PCR pattern (E13 and E14) were recovered from poultry cloacal swabs (PC 4 and PC 5) collected from LFC, Gannavaram. The discriminatory power ERIC-PCR and REP-PCR for P. mirabilis isolates was found to be 1 and 0.996, respectively. Close clustering between P. mirabilis of animal and human origin are indicative of probable zoonotic significance. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810113429 | |
| dc.keywords | β-LACTAMASES;WATER ;PROTEUS MIRABILIS;FOODS;ANIMAL ORIGIN, HUMAN;CLINICAL SAMPLES | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 150 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517502. (A.P.) INDIA | en_US |
| dc.sub | Veterinary Public Health | en_US |
| dc.subject | null | en_US |
| dc.theme | DETECTION OF PROTEUS MIRABILIS IN FOODS OF ANIMAL ORIGIN, ANIMAL AND HUMAN CLINICAL SAMPLES AND WATER WITH SPECIAL EMPHASIS ON β-LACTAMASES | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | DETECTION OF PROTEUS MIRABILIS IN FOODS OF ANIMAL ORIGIN, ANIMAL AND HUMAN CLINICAL SAMPLES AND WATER WITH SPECIAL EMPHASIS ON β-LACTAMASES | en_US |
| dc.type | Thesis | en_US |