DETECTION AND PARTIAL GENETIC CHARACTERISATION OF A NOVEL VARIANT OF AVIAN NEPHRITIS VIRUS IN INDIAN POULTRY FLOCKS SHOWING DIVERSE CLINICAL SIGNS
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Date
2015-06
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Abstract
Avian nephritis virus (ANV) infects poultry flocks worldwide, but no confirmed
cases have been reported from India so far. In the current study, disease
investigation was carried out in 21 broiler flocks at different parts of India with
clinical signs of nephritis, uneven and stunted growth, diarrhoea, reduced body
weight, and mortality up to 9.72%. Out of the 21 flocks screened, two were found
positive for ANV in RT-PCR assay. BLAST analysis revealed that the ANV of
Indian origin was closely related to ANV-1 strains reported from Japan, Hungary
and China. However, comparison of a small portion (~12% of nucleotides, i.e.
~60 nts, common site for ANV-1 and ANV-3, position 2200–2260 of ORF 1a
gene) of the Indian ANV sequence with ANV-3 sequences revealed 89–93%
identities with different ANV-3 isolates. Phylogenetically, ANV-1 forms three
clades, and the Indian ANV clustered under clade II. This study confirms the existence
of ANV in Indian poultry flocks and is the first report on the molecular detection
and genetic characterisation of ANV from India.
Description
TNV_AVH_2015_63(4)499-507
Keywords
Veterinary Science