MOLECULAR VARIABILITY AND INTEGRATED MANAGEMENT OF PAPAYA RINGSPOT VIRUS (PRSV)

dc.contributor.advisorNagaraju, N.
dc.contributor.authorANIL, PAPPACHAN
dc.date.accessioned2018-07-06T03:42:45Z
dc.date.available2018-07-06T03:42:45Z
dc.date.issued2017-09-28
dc.description.abstractPapaya Ringspot Virus (PRSV) is posing a major threat to papaya cultivation throughout India by rendering orchards economically unproductive. Survey conducted in 2014-15 revealed that 100 per cent PRSV incidence was observed at some locations of Bangalore rural and Bangalore urban districts of Karnataka, while highest average incidence was recorded at Ramanagara (82 %). In Andhra Pradesh, Kadapa district recorded highest average disease incidence of 70 per cent, while in Telangana, 24 per cent incidence of PRSV was observed in Hyderabad. In Kerala PRSV incidence was highest in Kottayam (77 %) and in Tamil Nadu, highest average incidence of 70 per cent was observed at Coimbatore. Comparison of P1 proteinase gene of PRSV deposited in NCBI GenBank revealed that nucleotide identity of South Indian PRSV isolates ranged from 87 to 72 per cent. None of the fifteen cultivated papaya varieties was found to possess resistance to PRSV both under glasshouse and field conditions. Extracts of Acorus calamus (5 %), Boerhavia diffusa (5 %), Kappaphycus alvarezii (KH-1 %), Eucheuma spinosum (SH-1 %) and Silicic acid (1 %) showed significant inhibitory effect on PRSV. Silver nanoparticles (60 to 100 nm) were prepared by green synthesis process using neem (Azadirachta indica) leaf extract. The extract of B. diffusa (5 %), colloidal Silver nanoparticles (100 ppm), Silver nanoparticles (50 ppm) prepared by green synthesis process and K. alvarezii (LBS3-1 %) showed significant inhibitory effect on PRSV multiplication both under field and glasshouse conditions. Adoption of integrated disease management module (III) a combination of maize (South African tall) as barrier crop, use of silver reflective mulch row cover and spraying with extract of A. calamus (5 %), K. alvarezii extract (KH-1 %) and insecticide imidacloprid (0.05 %) at monthly interval resulted in 142.39 per cent increase in yield over control with highest return per rupee invested (1.91).en_US
dc.identifier.otherTh-11854
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810057514
dc.language.isoenen_US
dc.pages197en_US
dc.publisherUNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURUen_US
dc.subPlant Pathologyen_US
dc.subjectnullen_US
dc.themeMOLECULAR VARIABILITY AND INTEGRATED MANAGEMENTen_US
dc.these.typePh.Den_US
dc.titleMOLECULAR VARIABILITY AND INTEGRATED MANAGEMENT OF PAPAYA RINGSPOT VIRUS (PRSV)en_US
dc.typeThesisen_US
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